SDS-22 stabilizes GSP-1/-2 PP1 subunits contributing to polarity establishment in <i>C. elegans</i> embryos

Article Article scientifique Article SDS-22 stabilizes GSP-1/-2 PP1 subunits contributing to polarity establishment in <i>C. elegans</i> embryos eng 2025-11-06 2025-12 author Li Yi 0009-0008-3904-8892 Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, 1211, Switzerland. 986659 Département de physiologie cellulaire et métabolisme (PHYME) author Calvi Ida 0000-0002-4416-5574 Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, 1211, Switzerland. 893006 author Gotta Monica 0000-0002-4336-7860 Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, 1211, Switzerland. monica.gotta@unige.ch. 659510 Département de physiologie cellulaire et métabolisme (PHYME) 6240-6265 10.1038/s44319-025-00624-0 41198903 1469-221X PMC12714725 https://link.springer.com/10.1038/s44319-025-00624-0 In many cells, polarity depends on the asymmetric distribution of the conserved PAR proteins, maintained by a balanced activity between kinases and phosphatases. The C. elegans one-cell embryo is polarized along the anterior-posterior axis, with the atypical protein kinase C PKC-3 enriched in the anterior, and the ring finger protein PAR-2 in the posterior. PAR-2 localization is regulated by PKC-3 and the PP1 phosphatases GSP-1/-2. Here we find that depletion of the conserved PP1 interactor SDS-22 leads to a partial rescue of the polarity defects of a pkc-3 temperature-sensitive mutant. Consistent with the rescue, SDS-22 depletion or mutation results in reduced GSP-1/-2 protein levels and activity. The decreased levels of GSP-1/-2 can be rescued by reducing proteasomal activity. Our data suggest that SDS-22 contributes to polarity by protecting the GSP-1 and GSP-2 catalytic subunits from proteasome-mediated degradation, supporting recent data in human cells showing that SDS22 is required to stabilize nascent PP1. Cell Polarity PAR Proteins PP1 Phosphatases Proteasomal Degradation SDS22 Dewey 612 NIH HHS P40 OD010440 Swiss National Science Foundation Polo like kinase 1 regulation and function in cell division and development 175850 CH 134 Département de physiologie cellulaire et métabolisme 21C false 509 La division asymétrique 847 https://yareta.unige.ch/archives/e77ecec3-fe36-4de8-8b35-14ca648710e5 EMBO reports 26 24 23513d17-4c41-4e25-8431-08d47f65fcd9 Article (Published version) Public Article_scientifique_from_crossref.pdf 5837871 application/pdf CC BY-4.0 4d7a40b2356480614ca5e5434c2f922c713b3104d2fb5b7c3630785f874335fb 17d533c1-8493-4cd6-8c5e-1efdb524c374 Supplemental data Public 44319_2025_624_MOESM1_ESM.pdf 3738086 application/pdf 188aa64ea97493eecc6b63b154dda445746f7103d09c11d329b0986a56607999 007110e0-af06-4e08-97cf-3ed4cc779eaa Supplemental data Public 44319_2025_624_MOESM2_ESM.pdf 994471 application/pdf e4b7a156f12ba612f908538f2fcce7677106de26e3dfbee29ebc345b742482e9 1a18d158-fbc0-47b6-bfb8-15766383002a Supplemental data Public 44319_2025_624_MOESM3_ESM.xlsx 15334 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet f3c3dc4fc17a1a7ee2b59ca2a3d7f2bd0a11eb2d007442621cc7df917eb8bbd8 c6700fc7-441c-45f7-b9ba-ae1251691985 Supplemental data Public 44319_2025_624_MOESM4_ESM.xlsx 23889 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet 3c8a97c100dcb7155b3ab647f080d53c84837315be00aaa8398ea2895d8eed67 c1ea1c8c-7789-493b-a6ef-4146bd5d9f97 Supplemental data Public 44319_2025_624_MOESM5_ESM.xlsx 20089 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet a04ddea93db00ca21675170c04a4e317f7f97ce53a6584643013e2c78e128e1e 4bcd8e31-2555-4c45-b68d-935eba1afac0 Supplemental data Public 44319_2025_624_MOESM6_ESM.zip 8209748 application/zip 8c6da8c5ae670f603cab4eab24b6879835fa03beabae391b9bd7335d542bdc4a 14f66494-9702-4e7f-b827-0633003ec9f6 Supplemental data Public 44319_2025_624_MOESM7_ESM.zip 3342640 application/zip edba175a8ee53e4205f7666c2559550aba2f4aaba360c651ce061b63c0f6a608 b050cd39-e1fa-4a4f-9de1-1547474a6d73 Supplemental data Public 44319_2025_624_MOESM8_ESM.zip 7764303 application/zip 22c899223f1e2c0f7f863bab8faff12cbcc6d356316d510aecdea04a478c80c5 8029af30-e738-475e-862a-7ca99894da0a Supplemental data Public 44319_2025_624_MOESM9_ESM.pdf 545085 application/pdf 75b6eb84c0f12912a3d747acd398543ef8cb2c52ecfcce0b19605dad6e0436f7 openAccess Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme

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SDS-22 stabilizes GSP-1/-2 PP1 subunits contributing to polarity establishment in C. elegans embryos	EMBO reports			2025	16	54

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