Transgenerational inheritance of centromere identity requires the CENP-A N-terminal tail in the C. elegans maternal germ line

Article Article scientifique Article Transgenerational inheritance of centromere identity requires the CENP-A N-terminal tail in the C. elegans maternal germ line eng 2021-07-06 2021-07-06 author Prosee Reinier 857221 author Wenda Joanna 0000-0002-7312-6777 848929 author Ozdemir Isa 0000-0002-6296-2403 935498 author Gabus Caroline 692546 author Delaney Kamila 0000-0002-1006-9146 841549 author Schwager Françoise 0000-0001-6923-1948 27468 author Gotta Monica 0000-0002-4336-7860 659510 author Steiner Florian 0000-0002-0514-5060 829674 e3000968 10.1371/journal.pbio.3000968 34228701 1544-9173 PMC8259991 https://dx.plos.org/10.1371/journal.pbio.3000968 <p> Centromere protein A (CENP-A) is a histone H3 variant that defines centromeric chromatin and is essential for centromere function. In most eukaryotes, CENP-A-containing chromatin is epigenetically maintained, and centromere identity is inherited from one cell cycle to the next. In the germ line of the holocentric nematode Caenorhabditis elegans , this inheritance cycle is disrupted. CENP-A is removed at the mitosis-to-meiosis transition and is reestablished on chromatin during diplotene of meiosis I. Here, we show that the N-terminal tail of CENP-A is required for the de novo establishment of centromeres, but then its presence becomes dispensable for centromere maintenance during development. Worms homozygous for a CENP-A tail deletion maintain functional centromeres during development but give rise to inviable offspring because they fail to reestablish centromeres in the maternal germ line. We identify the N-terminal tail of CENP-A as a critical domain for the interaction with the conserved kinetochore protein KNL-2 and argue that this interaction plays an important role in setting centromere identity in the germ line. We conclude that centromere establishment and maintenance are functionally distinct in C . elegans . </p> Dewey 570 Dewey 590 Dewey 612 Swiss National Science Foundation Regulation of centromeric chromatin 31003A_156774 CH Project funding Swiss National Science Foundation Regulation of centromeric chromatin 31003A_175606 CH Project funding 23 Institut de génétique et de génomique 0DH 43 Département de biologie moléculaire 161 134 Département de physiologie cellulaire et métabolisme 21C false 509 La division asymétrique 847 PLoS biology 19 7 25a56258-d1d5-4a58-973c-76261d5cd3d5 Article (Published version) Public Article-scientifique-from-unpaywall.pdf 3740937 application/pdf CC BY openAccess Centres et instituts / Institut de génétique et de génomique Faculté des sciences / Section de biologie / Département de biologie moléculaire Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme

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