Accumulation of Transcripts Encoding a Lipid Transfer-Like Protein During Deformation of Nodulation-Competent <i>Vigna unguiculata</i> Root Hairs

Article Article scientifique Article Accumulation of Transcripts Encoding a Lipid Transfer-Like Protein During Deformation of Nodulation-Competent <i>Vigna unguiculata</i> Root Hairs eng 1994 author Krause Andréa 24341 author Sigrist Christian 29612 author Dehning Irmtraut 915394 author Sommer Hans 63752 author Broughton William John 22724 411-418 10.1094/MPMI-7-0411 0894-0282 http://www.apsnet.org/publications/mpmi/backissues/Documents/1994Abstracts/Microbe07-411.htm A cDNA library was constructed from RNA of Vigna unguiculata root hairs harvested 1 day and 4 days after inoculation with Rhizobium sp. NGR234. A heterologous probe was used to identify a cDNA clone, the predicted 99-amino-acid sequence of which shares homology with a nonspecific lipid transfer protein (LTP) of Hordeum vulgare. Other characteristics, including an estimated molecular weight of 10.4 kD, an isoelectric point of 8.6, and a signal peptide with a hydrophobic region at the aminoterminal end, are shared by most LTPs. A transcript of 630 nt was found in all tissues tested, except nodules. Levels of mRNA increased in root hairs 24 hr after treatment with Rhizobium sp. NGR234, with different hormones, or with Nod factors. Amounts of transcripts were dependent on the concentration of Nod factors. Accumulation of transcripts during nodule development correlated with root hair deformation, the first visible step in the Rhizobium-legume symbiosis. Hadulin Root hair cDNA library Dewey 580 45 Département des sciences végétales 163 false Molecular Plant-Microbe Interactions 7 3 63879acb-1889-4d62-8c27-960ab95caf3b Article (Published version) Public Microbe07-411.pdf 1638905 application/pdf OA OTHER openAccess Faculté des sciences / Section de biologie / Département des sciences végétales

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Accumulation of Transcripts Encoding a Lipid Transfer-Like Protein During Deformation of Nodulation-Competent Vigna unguiculata Root Hairs	Molecular Plant-Microbe Interactions			1994	350	339

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Dehning, Irmtraut