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Scientific article
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English

Accumulation of Transcripts Encoding a Lipid Transfer-Like Protein During Deformation of Nodulation-Competent Vigna unguiculata Root Hairs

Published inMolecular Plant-Microbe Interactions, vol. 7, no. 3, p. 411-418
Publication date1994
Abstract

A cDNA library was constructed from RNA of Vigna unguiculata root hairs harvested 1 day and 4 days after inoculation with Rhizobium sp. NGR234. A heterologous probe was used to identify a cDNA clone, the predicted 99-amino-acid sequence of which shares homology with a nonspecific lipid transfer protein (LTP) of Hordeum vulgare. Other characteristics, including an estimated molecular weight of 10.4 kD, an isoelectric point of 8.6, and a signal peptide with a hydrophobic region at the aminoterminal end, are shared by most LTPs. A transcript of 630 nt was found in all tissues tested, except nodules. Levels of mRNA increased in root hairs 24 hr after treatment with Rhizobium sp. NGR234, with different hormones, or with Nod factors. Amounts of transcripts were dependent on the concentration of Nod factors. Accumulation of transcripts during nodule development correlated with root hair deformation, the first visible step in the Rhizobium-legume symbiosis.

Keywords
  • Hadulin
  • Root hair cDNA library
Citation (ISO format)
KRAUSE, Andréa et al. Accumulation of Transcripts Encoding a Lipid Transfer-Like Protein During Deformation of Nodulation-Competent <i>Vigna unguiculata</i> Root Hairs. In: Molecular Plant-Microbe Interactions, 1994, vol. 7, n° 3, p. 411–418. doi: 10.1094/MPMI-7-0411
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ISSN of the journal0894-0282
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