Decellularized Spinach Biomaterials Support Physiologically Relevant Mechanical Cyclic Strain and Prompt a Stretch-Induced Cellular Response

Article Article scientifique Article Decellularized Spinach Biomaterials Support Physiologically Relevant Mechanical Cyclic Strain and Prompt a Stretch-Induced Cellular Response eng 2022-11-11 2022-12-19 author Harris Ashlee F. 0000-0002-2049-3397 author Lacombe Jerome author Sanchez-Ballester Noelia M. author Victor Shaun author Curran Killian Anthony Joseph 879498 author Nordquist Alan R. author Thomas Baiju author Gu Jian author Veuthey Jean-Luc 22317 author Soulairol Ian author Zenhausern Frederic 5682-5692 10.1021/acsabm.2c00721 36368008 2576-6422 https://pubs.acs.org/doi/10.1021/acsabm.2c00721 Recently, decellularized plant biomaterials have been explored for their use as tissue engineered substitutes. Herein, we expanded upon the investigation of the mechanical properties of these materials to explore their elasticity as many anatomical areas of the body require biomechanical dynamism. We first constructed a device to secure the scaffold and induce a strain within the physiological range of the normal human adult lung during breathing (12–20 movements/min; 10–20% elongation). Results showed that decellularized spinach leaves can support cyclic strain for 24 h and displayed heterogeneous local strain values (7.76–15.88%) as well as a Poisson’s ratio (0.12) similar to that of mammalian lungs (10.67–19.67%; 0.01), as opposed to an incompressible homogeneous standard polymer (such as PDMS (10.85–12.71%; 0.4)). Imaging and mechanical testing showed that the vegetal scaffold exhibited strain hardening but maintained its structural architecture and water retention capacity, suggesting an unaltered porosity. Interestingly, we also showed that cells seeded on the scaffold can also sense the mechanical strain as demonstrated by a nuclear reorientation perpendicular to strain direction (63.3° compared to 41.2° for nonstretched cells), a nuclear location of YAP and increased expression of YAP target genes, a high cytoplasmic calcium level, and an elevated expression level of collagen genes (COL1A1, COL3A1, COL4A1, and COL6A) with an increased collagen secretion at the protein level. Taken together, these data demonstrated that decellularized plant leaf tissues have an inherent elastic property similar to that found in the mammalian system to which cells can sense and respond. Biomaterial Decellularization Elongation Lung Mechanotransduction Plant-based scaffolds Stretching Tissue engineering Dewey 615 31 Section des sciences pharmaceutiques 17 false 881 Sciences analytiques ACS applied bio materials 5 12 4bb0cdb3-ab85-402f-961b-712f9755f7fc Article (Published version) Restricted Article-scientifique-from-crossref.pdf 6592363 application/pdf bcd0e9dd-f785-4481-b042-5b7335e3fee7 Appendix Public mt2c00721_si_001.pdf 384243 application/pdf Vignette Public images_large_mt2c00721_0005.jpeg 171047 image/jpeg restrictedAccess Faculté des sciences / Section des sciences pharmaceutiques

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Decellularized Spinach Biomaterials Support Physiologically Relevant Mechanical Cyclic Strain and Prompt a Stretch-Induced Cellular Response	ACS applied bio materials			2022	144	2

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Curran, Killian Anthony Joseph