Upstream Regulation of TORC2 Signalling

Diplôme Thèse Upstream Regulation of TORC2 Signalling eng 2021-03-19 author Stankova Monika 859035 director Loewith Robbie Joséph 630703 10.13097/archive-ouverte/unige:151982 urn:nbn:ch:unige-1519826 Sc. Vie 98 The Target Of Rapamycin (TOR) kinase functions in two unique protein complexes, named TORC1 and TORC2. TORC1 and TORC2 differ in terms of their structure and localization, but they also present some similarities including the upstream stimuli they sense; i.e. both complexes appear to respond to changes in glucose levels. We found that TORC2 senses glucose in a manner that is at least partially distinct from the glucose signalling that is upstream of TORC1 which involves the SEA and EGO complexes. In contrast, our results demonstrate that the first step in glucose signalling to TORC2 requires any of the three yeast hexokinases bound to their substrate. We genetically uncoupled the catalytic and signalling function of hexokinases, further supporting the role of these glycolytic enzymes in glucose signalling per se. Interestingly, we found that this signalling pathway acts in parallel to inversely regulate the yeast AMPK - Snf1. TOR signalling Glucose Budding yeast Dewey 570 43 Département de biologie moléculaire 161 false 814 Groupe Loewith 56a103ce-a617-468f-b358-75c622bc8d13 Thesis Restricted PhDthesis_MonikaStankova.pdf 3407732 application/pdf Private 2023-05-19 b51193bd6d55949ad734cf1846b6f4af5d3eb7e46a5b052767e33337ee6ccc8b 9b78d15f-14d6-4f61-abdd-e8be53cd202d Agreement Private Stankova-Monika_Contrat_diffusion_t.pdf 486709 application/pdf 1b48c9322a02ad93d7a64f7c81e80e5ed644ffbea185600623321c21838c9bd9 33399928-c65a-4f0f-9361-3361234134ca Publication mode Private Stankova-Monika_Mode-de-publication.pdf 482929 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire

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Upstream Regulation of TORC2 Signalling				2021	389	4

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Stankova, Monika