Age dependence of human gastrocnemius Mg2+: fitting 31P-NMR spectra using quantum mechanics-based prior knowledge

Article Article scientifique Article Age dependence of human gastrocnemius Mg2+: fitting 31P-NMR spectra using quantum mechanics-based prior knowledge eng 2001 author Binzoni Tiziano 0009-0004-2592-7926 23000 author Ngo Loan 527028 author Janssens Marina Anne Béatrice 84061 author Hiltbrand Emile 668277 author Bianchi Stefano 82133 author Cerretelli Paolo 25401 author Graveron-Demilly D 275-283 10.2114/jpa.20.275 11759266 1345-3475 The age dependence of human gastrocnemius Mg2+ concentration is demonstrated. To quantitate Mg2+ concentration, an original and accurate fitting algorithm using quantum mechanics-based prior knowledge is detailed. In a group of 28 volunteers (14 females) in the age range 5-80 years, pH, PCr/ATP and Pi/ATP values in the gastrocnemius were 7.02 +/- 0.02 pH, 4.16 +/- 0.33 and 0.13 +/- 0.02, respectively and independent of age and sex. By contrast, intracellular Mg2+ concentration (mM) decreased linearly (p < 0.05) with age (Mg2+ = 0.7803 +/- 0.0247-0.0027 +/- 0.0005 * age). No difference was found between sexes. From these results, it follows that care must be taken when comparing muscle Mg2+ data from subjects of different age. The hypothesis can be put forward that during aging insufficient intake and/or increased depletion of Mg2+ (e.g., intestinal hypoabsorption or urinary leakage) may affect the musculoskeletal system. Adenosine Triphosphate/metabolism Adolescent Adult Age Factors Aged Aged, 80 and over Algorithms Child Child, Preschool Computer Simulation Female Humans Hydrogen-Ion Concentration Magnesium/metabolism/physiology Male Middle Aged Monte Carlo Method Muscle, Skeletal/metabolism Nuclear Magnetic Resonance, Biomolecular/methods Phosphates/metabolism Phosphocreatine/metabolism Phosphorus Quantum Theory Dewey 616.0757 Dewey 616.8 53 Département de neurosciences fondamentales 21A 62 Département de radiologie et informatique médicale 227 false 184 Groupe Binzoni Tiziano (radiologie) 234 Journal of physiological anthropology and applied human science 20 5 eab610fa-60d7-4802-9fd7-094bffef8293 Article (Published version) Restricted 20_275.pdf 214225 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de neurosciences fondamentales Faculté de médecine / Section de médecine clinique / Département de radiologie et informatique médicale

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Age dependence of human gastrocnemius Mg2+: fitting 31P-NMR spectra using quantum mechanics-based prior knowledge	Journal of physiological anthropology and applied human science			2001	457	0

Archive ouverte UNIGE

Janssens, Marina Anne Béatrice