Role of the chromatin organization in transcription termination

Diplôme Master Role of the chromatin organization in transcription termination eng 2022-05-02 author Renaudineau Solène 837588 director Soudet Julien 824053 director Stutz Françoise 64881 Transcription is a process by which the RNA is produced based on the information contained in the DNA. In eukaryotic organisms, this fundamental step of gene expression takes place in the nucleus. Although transcription initiation is more or less understood, transcription termination remains unclear. The most accepted theory suggests that the RNA polymerase 2 (RNAPII) is detached from the DNA matrix via the action of an exo-ribonuclease. However, it seems that this enzyme is not sufficient for a complete termination. An RNAPII pause would also be necessary to promote interaction between the polymerase and the ribonuclease. Yet how RNAPII is stopped at the end of the gene is not clearly defined. Transcription termination also includes cleavage and polyadenylation of the transcript. This step depends on a variety of factors recruited by the phosphorylated residues present on the carboxy-terminal domain (CTD) of the polymerase. Nevertheless, the mechanism triggering the phosphorylation of these specific amino acids is not known. In this study, we propose a link between chromatin conformation and transcription termination. Since transcription appears to terminate in a nucleosome depleted region, we asked whether the sudden change in chromatin structure could be responsible of CTD phosphorylation. Moreover, as this region is accessible to many transcription factors, we tried to address whether the polymerase could be stopped by one of these proteins, or by a nucleosome following the terminal nucleosome depleted region (NDR). We finally propose a new model linking chromatin conformation to elements essential for efficient transcription termination. Dewey 570 Maîtrise universitaire en biologie 46 Département de biologie cellulaire 165 false 839 Groupe Stutz b83769a5-6c38-46ba-a2c1-53de96f5275f Master thesis Restricted Mémoire solène renaudineau[1].pdf 2356834 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département de biologie cellulaire

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Role of the chromatin organization in transcription termination				2022	54	7

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Renaudineau, Solène

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