Interleukin 12 in the acute phase of the immune response after excimer laser treatment

Article Article scientifique Article Interleukin 12 in the acute phase of the immune response after excimer laser treatment eng 2023-07-04 2023-07-04 author Resan Mirko author Cvejic Zeljka 0000-0002-8218-4899 author Pancevski Igor 0000-0001-6382-8161 author Thumann Gabriele 0000-0003-3682-9569 785873 Département des neurosciences cliniques (OTONO) author Kropp Martina 0000-0001-6861-5851 793095 author Guber Ivo 0000-0002-5125-8840 927275 author Ristic Dragana 836533 author Vojvodic Danilo 0000-0001-9308-3076 author Pajic Bojan 0000-0003-1987-6232 820586 Département des neurosciences cliniques (OTONO) 4472 10.3390/jcm12134472 37445506 2077-0383 PMC10342327 https://www.mdpi.com/2077-0383/12/13/4472 <p>Background and objectives: The aim of the research was to investigate the differences in the concentrations of IL-12, IL-4, IL-10, and IFN-γ in tears after LASIK and PRK procedures. Materials and methods: The study included 68 myopic eyes up to −3.0 D refractive spherical equivalent, divided into two groups: Group 1 LASIK (n = 31) and Group 2 PRK (n = 37). Three tear samples were taken from each eye: immediately before the procedure (t0), 1 h after the procedure (t1), and 24 h after the procedure (t2). The concentrations of IL-12p70, IL-4, IL-10, and IFN-γ in the tear samples were determined by flow cytometry. Participants were not taking anti-inflammatory therapy 24 h after the procedure. Results: IL-4 levels 1 h after treatment did not differ between LASIK and PRK (p = 0.990), while 24 h after PRK there was a significant decrease in IL-4 levels (p < 0.05), but not after LASIK (p = 0.476). In both the LASIK (p < 0.05) and PRK (p < 0.05) groups, there is an increase in IL-10 concentrations 1 h after treatment, which persists 24 h after LASIK (p < 0.05) but not after PRK (p = 0.081). There is an increase in IL-12p70 concentration 1 h after treatment in both the LASIK (p < 0.001) and PRK groups (p < 0.001). There is also an increase in IL-12p70 concentration 24 h after PRK (p < 0.005), but not after LASIK (p = 0.775). Conclusions: IL-4 concentration shows a significantly higher value in the LASIK group than in the PRK group after 24 h. IL-10 and IL-12p70 levels increase one hour after surgery in both groups. After 24 h, the IL-10 levels remain elevated in the LASIK group, and the IL-12p70 levels remain elevated in the PRK group. Thus, LASIK and PRK procedures show different inflammatory dynamics.</p> IL-10 IL-12 IL-4 LASIK PRK Corneal wound healing Excimer laser Tears Dewey 616.8 61 Département des neurosciences cliniques 226 false 582 Ophtalmologie expérimentale 925 Journal of clinical medicine 12 13 dd33687b-f5a9-4417-973e-5781783aa52b Article (Published version) Public Article-scientifique-from-unpaywall.pdf 1723628 application/pdf CC BY-4.0 e9c050161742b69a9d76a35cc0b2d248e44c47a0e948f8d3f5b4d91cfed83123 openAccess Faculté de médecine / Section de médecine clinique / Département des neurosciences cliniques

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Interleukin 12 in the acute phase of the immune response after excimer laser treatment	Journal of clinical medicine			2023	98	107

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