Scientific article
Open access

Interleukin 12 in the acute phase of the immune response after excimer laser treatment

Published inJournal of clinical medicine, vol. 12, no. 13, 4472
Publication date2023-07-04
First online date2023-07-04

Background and objectives: The aim of the research was to investigate the differences in the concentrations of IL-12, IL-4, IL-10, and IFN-γ in tears after LASIK and PRK procedures. Materials and methods: The study included 68 myopic eyes up to −3.0 D refractive spherical equivalent, divided into two groups: Group 1 LASIK (n = 31) and Group 2 PRK (n = 37). Three tear samples were taken from each eye: immediately before the procedure (t0), 1 h after the procedure (t1), and 24 h after the procedure (t2). The concentrations of IL-12p70, IL-4, IL-10, and IFN-γ in the tear samples were determined by flow cytometry. Participants were not taking anti-inflammatory therapy 24 h after the procedure. Results: IL-4 levels 1 h after treatment did not differ between LASIK and PRK (p = 0.990), while 24 h after PRK there was a significant decrease in IL-4 levels (p < 0.05), but not after LASIK (p = 0.476). In both the LASIK (p < 0.05) and PRK (p < 0.05) groups, there is an increase in IL-10 concentrations 1 h after treatment, which persists 24 h after LASIK (p < 0.05) but not after PRK (p = 0.081). There is an increase in IL-12p70 concentration 1 h after treatment in both the LASIK (p < 0.001) and PRK groups (p < 0.001). There is also an increase in IL-12p70 concentration 24 h after PRK (p < 0.005), but not after LASIK (p = 0.775). Conclusions: IL-4 concentration shows a significantly higher value in the LASIK group than in the PRK group after 24 h. IL-10 and IL-12p70 levels increase one hour after surgery in both groups. After 24 h, the IL-10 levels remain elevated in the LASIK group, and the IL-12p70 levels remain elevated in the PRK group. Thus, LASIK and PRK procedures show different inflammatory dynamics.

  • IL-10
  • IL-12
  • IL-4
  • PRK
  • Corneal wound healing
  • Excimer laser
  • Tears
Citation (ISO format)
RESAN, Mirko et al. Interleukin 12 in the acute phase of the immune response after excimer laser treatment. In: Journal of clinical medicine, 2023, vol. 12, n° 13, p. 4472. doi: 10.3390/jcm12134472
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Article (Published version)
ISSN of the journal2077-0383

Technical informations

Creation10/10/2023 9:42:09 AM
First validation02/20/2024 7:32:45 AM
Update time02/20/2024 7:32:45 AM
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