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Scientific article
Open access
English

Interleukin 12 in the acute phase of the immune response after excimer laser treatment

Published inJournal of clinical medicine, vol. 12, no. 13, 4472
Publication date2023-07-04
First online date2023-07-04
Abstract

Background and objectives: The aim of the research was to investigate the differences in the concentrations of IL-12, IL-4, IL-10, and IFN-γ in tears after LASIK and PRK procedures. Materials and methods: The study included 68 myopic eyes up to −3.0 D refractive spherical equivalent, divided into two groups: Group 1 LASIK (n = 31) and Group 2 PRK (n = 37). Three tear samples were taken from each eye: immediately before the procedure (t0), 1 h after the procedure (t1), and 24 h after the procedure (t2). The concentrations of IL-12p70, IL-4, IL-10, and IFN-γ in the tear samples were determined by flow cytometry. Participants were not taking anti-inflammatory therapy 24 h after the procedure. Results: IL-4 levels 1 h after treatment did not differ between LASIK and PRK (p = 0.990), while 24 h after PRK there was a significant decrease in IL-4 levels (p < 0.05), but not after LASIK (p = 0.476). In both the LASIK (p < 0.05) and PRK (p < 0.05) groups, there is an increase in IL-10 concentrations 1 h after treatment, which persists 24 h after LASIK (p < 0.05) but not after PRK (p = 0.081). There is an increase in IL-12p70 concentration 1 h after treatment in both the LASIK (p < 0.001) and PRK groups (p < 0.001). There is also an increase in IL-12p70 concentration 24 h after PRK (p < 0.005), but not after LASIK (p = 0.775). Conclusions: IL-4 concentration shows a significantly higher value in the LASIK group than in the PRK group after 24 h. IL-10 and IL-12p70 levels increase one hour after surgery in both groups. After 24 h, the IL-10 levels remain elevated in the LASIK group, and the IL-12p70 levels remain elevated in the PRK group. Thus, LASIK and PRK procedures show different inflammatory dynamics.

eng
Keywords
  • IL-10
  • IL-12
  • IL-4
  • LASIK
  • PRK
  • Corneal wound healing
  • Excimer laser
  • Tears
Citation (ISO format)
RESAN, Mirko et al. Interleukin 12 in the acute phase of the immune response after excimer laser treatment. In: Journal of clinical medicine, 2023, vol. 12, n° 13, p. 4472. doi: 10.3390/jcm12134472
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ISSN of the journal2077-0383
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Creation10/10/2023 9:42:09 AM
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