Deciphering the origins and fates of steroidogenic lineages in the mouse testis

Article Article scientifique Article Deciphering the origins and fates of steroidogenic lineages in the mouse testis eng 2022-06-14 author Ademi Herta 834331 author Djari Cyril Claude Michel 955572 author Mayere Chloé 0000-0002-9254-4886 889663 author Neirijnck Yasmine 834332 author Sararols Pauline 0000-0001-8063-1096 883030 author Rands Christopher 850350 author Stevant Isabelle 805551 author Conne Béatrice 117948 author Nef Serge 0000-0001-5462-0676 29062 110935 10.1016/j.celrep.2022.110935 35705036 2211-1247 Leydig cells (LCs) are the major androgen-producing cells in the testis. They arise from steroidogenic progenitors (SPs), whose origins, maintenance, and differentiation dynamics remain largely unknown. Single-cell transcriptomics reveal that the mouse steroidogenic lineage is specified as early as embryonic day 12.5 (E12.5) and has a dual mesonephric and coelomic origin. SPs specifically express the Wnt5a gene and evolve rapidly. At E12.5 and E13.5, they give rise first to an intermediate population of pre-LCs, and finally to fetal LCs. At E16.5, SPs possess the characteristics of the dormant progenitors at the origin of adult LCs and are also transcriptionally closely related to peritubular myoid cells (PMCs). In agreement with our in silico analysis, in vivo lineage tracing indicates that Wnt5a-expressing cells are bona fide progenitors of PMCs as well as fetal and adult LCs, contributing to most of the LCs present in the fetal and adult testis. CP: Developmental biology Leydig cells WNT5A In vivo lineage tracing Peritubular myoid cells ScRNA-seq Single-cell RNA sequencing Steroidogenic progenitors Testis Androgens Animals Cell Differentiation Fetus Leydig Cells Male Mice Testis Dewey 590 Dewey 576.5 Swiss National Science Foundation Revisiting adreno-gonadal development and cell fate commitment using single cell RNA sequencing 31003A_173070 CH Project funding Swiss National Science Foundation Investigating gonadal sex differentiation by single cell transcriptomics in mice and humans 310030_200316 CH Project funding 135 Département de médecine génétique et développement 21D 23 Institut de génétique et de génomique 0DH false 448 Mécanismes moléculaires et génétiques du développement sexuel 778 Cell reports 39 11 9736109c-968c-49ac-8172-9fb959b92bee Article (Published version) Public Article-scientifique-from-unpaywall.pdf 6277151 application/pdf CC BY-NC-ND-4.0 c120210b-20c5-4bbf-be9b-ef27b9f07ba6 Supplemental data Public 1-s2.0-S2211124722007173-mmc1.pdf 3370922 application/pdf CC BY-NC-ND-4.0 c9f639ab-495d-44e3-a7dc-b4997a793721 Supplemental data Public 1-s2.0-S2211124722007173-mmc2.xlsx 436635 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet CC BY-NC-ND-4.0 c81d0792-eb99-4d9c-82cb-e5356d289875 Supplemental data Public 1-s2.0-S2211124722007173-mmc3.xlsx 1466923 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet CC BY-NC-ND-4.0 3ae1f019-1ee4-42e5-b92d-b85c41f1e4a4 Supplemental data Public 1-s2.0-S2211124722007173-mmc4.pdf 9650404 application/pdf CC BY-NC-ND-4.0 openAccess Faculté de médecine / Section de médecine fondamentale / Département de médecine génétique et développement Centres et instituts / Institut de génétique et de génomique

Title	Published in	Year	Views	Downloads

Deciphering the origins and fates of steroidogenic lineages in the mouse testis	Cell reports	2022	248	225
The Wnt5aTet-ON 3G mouse model reveals new insights on the steroidogenic cell lineage		2020	524	0

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