Determination of amphetamines by high-performance liquid chromatography with ultraviolet detection: on-line pre-column derivatization with 9-fluorenylmethyl chloroformate and preconcentration

Article Article scientifique Article Determination of amphetamines by high-performance liquid chromatography with ultraviolet detection: on-line pre-column derivatization with 9-fluorenylmethyl chloroformate and preconcentration eng 1992 author Maeder Gabrielle Ruth 79002 author Pelletier Michel 509508 author Haerdi Werner 24913 Administration de la faculté des sciences (SIAD) 9-14 10.1016/0021-9673(92)80258-V 0021-9673 An on-line pre-column derivatization method for the determination of low concentrations of amphetamines using ultraviolet detection has been developed. In this work 9-fluorenylmethyl chloroformate (FMOC-Cl) has been used as the derivatizing agent. The optimum conditions for derivatization such as pH, reaction time and FMOC-Cl/amphetamine concentration ratio have been investigated. Attempts have been made to extend the sensitivity of the method by preconcentration of the derivatives on a micro-column packed with C18 bonded silica. Derivatization and preconcentration of the samples were carried out at low pressure on a flow injection analysis system. Quantitative determination of amphetamines as low as 2 • 10-8 mol/l can be made using this on-line method with preconcentration. The sensitivity of this technique is about 50 times greater the equivalent off-line method. Dewey 540 Dewey 614.1 40 Département de chimie minérale, analytique et appliquée 145 67 Institut de médecine légale 22A2 false Journal of chromatography 593 224dbd6b-83f1-481a-883c-bea7c56f5b7c Article (Published version) Restricted MAEDER_JOC_199214.pdf 2509454 application/pdf f5003167d3bac1b263bffb6dfbb0deff44a5294efffadf140554c64f00362ff2 restrictedAccess Faculté des sciences / Section de chimie et biochimie / Département de chimie minérale, analytique et appliquée Faculté de médecine / Section de médecine clinique / Département de santé et médecine communautaires / Institut de médecine légale

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Determination of amphetamines by high-performance liquid chromatography with ultraviolet detection: on-line pre-column derivatization with 9-fluorenylmethyl chloroformate and preconcentration	Journal of chromatography			1992	84	0

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Maeder, Gabrielle Ruth