Quantification of titanium and zirconium elements in oral mucosa around healthy dental implants : a case-control pilot study

Article Article scientifique Article Quantification of titanium and zirconium elements in oral mucosa around healthy dental implants : a case-control pilot study eng 2023-06-04 2023-08 author Cionca Norbert Division of Regenerative Dental Medicine and Periodontology, University Clinics of Dental Medicine, University of Geneva, Geneva, Switzerland. Norbert.cionca@unige.ch. 508673 CUMD - Clin. méd.dent.régénérative & parodontologie (CPAR) author Meyer Julien Mass Spectrometry Core Facility (MZ 2.0), Faculty of Sciences, University of Geneva, Geneva, Switzerland. 769322 Services techniques communs (SETCO) author Michalet Sophie Mass Spectrometry Core Facility (MZ 2.0), Faculty of Sciences, University of Geneva, Geneva, Switzerland. 707254 Administration de la faculté des sciences (SIAD) author Varesio Emmanuel Mass Spectrometry Core Facility (MZ 2.0), Faculty of Sciences, University of Geneva, Geneva, Switzerland. 30230 Section des sciences pharmaceutiques (FARMA) author Hashim Dena Talal Division of Regenerative Dental Medicine and Periodontology, University Clinics of Dental Medicine, University of Geneva, Geneva, Switzerland. 749430 CUMD - Clin. méd.dent.régénérative & parodontologie (CPAR) 4715-4726 10.1007/s00784-023-05099-8 37270723 1432-6981 PMC10415439 https://link.springer.com/article/10.1007/s00784-023-05099-8 Objectives: Metallic particles are detected in different sites of the oral cavity, mainly in patients with peri-implantitis lesions. The aim of this pilot study was to analyze the levels of titanium and zirconium elements in the oral mucosa around healthy implants and to investigate the impact of titanium exogenous contamination on the measurements. Materials and methods: Forty-one participants were included in this three-phase study. Two groups of subjects were defined according to presence of titanium or zirconia implants (n: 20) or without any implants nor metallic restorations (n:21). Thirteen patients (n: 5 with zirconia implant; n: 3 with titanium implants; n: 5 control group) took part to the first part designed to optimize and validate the method of detecting titanium (Ti) and zirconium (Zr) elements in the oral mucosa and gingival tissues by the Inductively Coupled Plasma Mass Spectrometry (ICPMS). The second phase compared the levels of Ti and Zr concentrations in patients with implants (n: 12) and without implants (n: 6) who were controlled for their intake of titanium dioxide (TiO2). The last step included ten control subjects without any metallic devices to measure the concentration of Ti and Zr before and after having candies containing TiO2. Results: In the first phase, concentrations of Ti and Zr were below the limit of detection (LOD) in most cases, 0.18 μg/L and 0.07 μg/L respectively. In the titanium group, two out of three subjects displayed concentrations above the LOD, 0.21 μg/L and 0.66 μg/L. Zr element was only found in patients with zirconia implants. After controlling the intake of TiO2, all concentrations of Ti and Zr were below the limit of quantification (LOQ). Moreover, in patients with no implants, the Ti concentration in gingiva cells was superior for 75% of the samples after having a TiO2 diet. Conclusions: Zirconium was only found in patients with zirconia implants, whereas titanium was detected in all groups even in subjects with no titanium implants. Zirconium and titanium elements were not detected in patients who were controlled for their intake of food and their use of toothpaste irrespective of the presence of implants or not. For 70% of the patients, the titanium detection was directly influenced by the intake of TiO2 contained candies. Clinical relevance: When analyzing titanium particles, it is necessary to pay attention to the risk of contamination bias brought by external products. When this parameter was controlled, no titanium particles were detected around clinically healthy implants. Case–control Dental implants Spectrophotometry 3 Titanium 1 Zirconium 2 Humans Mouth Mucosa / pathology Dental Implants / adverse effects Zirconium Pilot Projects Dewey 617.6 Dewey 500 206 Division de paradontologie 24P2 2 Faculté des sciences 1 false 360 Groupe Giannopoulou Catherine (médecine dentaire) 626 Clinical oral investigations 27 8 4312c76a-5621-47c8-add3-119bcc9448aa Article (Published version) Public Article_scientifique_from_unpaywall.pdf 1292896 application/pdf CC BY-4.0 547f12520a78898a3497f2f0a9820421c260985eae1271ae0f5bcf6c1309dc5e openAccess Faculté de médecine / Section de médecine dentaire / Département de médecine dentaire préventive et de premier recours / Division de paradontologie Faculté des sciences

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Quantification of titanium and zirconium elements in oral mucosa around healthy dental implants : a case-control pilot study	Clinical oral investigations			2023	82	203

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Meyer, Julien