A covalently linked probe to monitor local membrane properties surrounding plasma membrane proteins

Article Article scientifique Article A covalently linked probe to monitor local membrane properties surrounding plasma membrane proteins eng 2022-12-26 2023-03-06 author Umebayashi Miwa 0000-0002-5873-8417 Department of Biochemistry and National Centre for Competence in Research in Chemical Biology, Sciences II, University of Geneva, Geneva, Switzerland.,Myoridge Co. Ltd., Kyoto, Japan. 691889 author Takemoto Satoko 0000-0003-0258-6589 Image Processing Research Team, RIKEN Centre for Advanced Photonics, Wako, Japan. author Reymond Luc Michel 0000-0001-5282-9273 Ecole Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, National Centre of Competence in Research (NCCR) in Chemical Biology, Lausanne, Switzerland. 856178 author Sundukova Mayya 0000-0003-1328-0008 Epigenetics and Neurobiology Unit, European Molecular Biology Laboratory Rome, Monterotondo, Italy.,Instituto Biofisika (UPV/EHU, CSIC), University of the Basque Country, Leioa, Spain.,Fundación Biofísica Bizkaia/Biofisika Bizkaia Fundazioa (FBB), Leioa, Spain. author Hovius Ruud 0000-0001-9258-6587 Ecole Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, National Centre of Competence in Research (NCCR) in Chemical Biology, Lausanne, Switzerland. 403525 author Bucci Annalisa 0000-0002-6955-2204 Epigenetics and Neurobiology Unit, European Molecular Biology Laboratory Rome, Monterotondo, Italy. author Heppenstall Paul A 0000-0001-7954-5166 Epigenetics and Neurobiology Unit, European Molecular Biology Laboratory Rome, Monterotondo, Italy. author Yokota Hideo 0000-0003-1395-309X Image Processing Research Team, RIKEN Centre for Advanced Photonics, Wako, Japan. author Johnsson Kai 0000-0002-8002-1981 Department of Chemical Biology, Max Planck Institute for Medical Research, Heidelberg, Germany. 742114 author Riezman Howard 0000-0003-4680-9422 Department of Biochemistry and National Centre for Competence in Research in Chemical Biology, Sciences II, University of Geneva, Geneva, Switzerland. 134430 Département de biochimie (CHIBI) 1-27 e202206119 10.1083/jcb.202206119 36571579 0021-9525 PMC9802683 https://rupress.org/jcb/article/222/3/e202206119/213783/A-covalently-linked-probe-to-monitor-local Functional membrane proteins in the plasma membrane are suggested to have specific membrane environments that play important roles to maintain and regulate their function. However, the local membrane environments of membrane proteins remain largely unexplored due to the lack of available techniques. We have developed a method to probe the local membrane environment surrounding membrane proteins in the plasma membrane by covalently tethering a solvatochromic, environment-sensitive dye, Nile Red, to a GPI-anchored protein and the insulin receptor through a flexible linker. The fluidity of the membrane environment of the GPI-anchored protein depended upon the saturation of the acyl chains of the lipid anchor. The local environment of the insulin receptor was distinct from the average plasma membrane fluidity and was quite dynamic and heterogeneous. Upon addition of insulin, the local membrane environment surrounding the receptor specifically increased in fluidity in an insulin receptor-kinase dependent manner and on the distance between the dye and the receptor. Biochemistry Membrane and lipid biology Dewey 540 Leducq Foundation Swiss National Science Foundation NCCR Chemical Biology - Visualisation and Control of Biological Processes Using Chemistry (phase III) 185898 CH Swiss National Science Foundation Function and regulation of sphingolipids and ether lipids 184949 CH 39 Département de biochimie 144 false 768 Groupe Riezman 860 NCCR Chemical Biology The Journal of cell biology 222 3 5ed4d62f-6c83-4607-84d3-c9329ada3396 Article (Published version) Public jcb_202206119_Copy.pdf 6761603 application/pdf CC BY-NC-SA-4.0 fabe8aa25d08090539ca77bbd601e71bb970363787dcdc412df70973c950f77e openAccess Faculté des sciences / Section de chimie et biochimie / Département de biochimie

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