Anti-protein S antibodies following a varicella infection: detection, characterization and influence on thrombin generation

Article Article scientifique Rapport de cas Anti-protein S antibodies following a varicella infection: detection, characterization and influence on thrombin generation eng 2005 author Regnault V author Boehlen Françoise 33004 author Ozsahin Ayse Hulya 121635 author Wahl Denys 734470 author de Groot PG author Lecompte Thomas Pierre 768575 author De Moerloose Philippe 26335 1243-1249 10.1111/j.1538-7836.2005.01270.x 15946215 1538-7836 Postinfectious purpura fulminans is a rare disease. Varicella is one of the precipitating conditions and we recently observed such a case. The 4-year-old child was found to have a severe transient protein S deficiency. By enzyme-linked immunosorbent assay and surface plasmon resonance we first demonstrated that anti-protein S antibodies were present and also transient. Next we characterized the epitopes against which these antibodies were directed and found that they predominantly recognized the N-terminal part of protein S. Finally we showed by thrombography a transient dramatic hypercoagulable state as a result of thrombin being unregulated by the dynamic protein C inhibitory system: in vitro thrombin generation, in response to a low concentration of tissue factor, was almost insensitive to activated protein C up to 25 nmol L(-1) on day 4 while it was normally sensitive on day 42. For the first time, we demonstrated a temporal relationship between protein S deficiency, antibodies to protein S and hypercoagulability, thus supporting the pathogenic role of these antibodies. Autoantibodies/blood Chickenpox/blood/complications Child, Preschool Enzyme-Linked Immunosorbent Assay Epitope Mapping Humans Male Protein S/immunology Protein S Deficiency/etiology/virology Purpura, Schoenlein-Henoch/etiology/virology Surface Plasmon Resonance Thrombin/biosynthesis Thrombophilia/etiology/virology Time Factors Dewey 616 Dewey 618 56 Département de médecine 221 58 Département de pédiatrie, gynécologie et obstétrique 223 false 10 Anticorps antiphospholipides 12 Journal of thrombosis and haemostasis 3 6 7bfc28bb-58dc-4739-a613-3e90accca32e Article (Published version) Restricted 1243-1249.pdf 474208 application/pdf restrictedAccess Faculté de médecine / Section de médecine clinique / Département de médecine Faculté de médecine / Section de médecine clinique / Département de pédiatrie, gynécologie et obstétrique

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Anti-protein S antibodies following a varicella infection: detection, characterization and influence on thrombin generation	Journal of thrombosis and haemostasis			2005	488	0

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Wahl, Denys