Evaluation and comparison of various separation techniques for the analysis of closely-related compounds of pharmaceutical interest

Article Article scientifique Article Evaluation and comparison of various separation techniques for the analysis of closely-related compounds of pharmaceutical interest eng 2013 author Gourmel Charlotte 633588 author Grand-Guillaume-Perrenoud Alexandre 631334 author Waller Laura 696055 author Reginato Emilie 661508 author Verne Joelle author Dulery Bertrand author Veuthey Jean-Luc 22317 author Rudaz Serge 0000-0002-4180-5417 28978 author Schappler Julie 506604 author Guillarme Davy 623637 172-177 10.1016/j.chroma.2013.01.095 0021-9673 http://linkinghub.elsevier.com/retrieve/pii/S0021967313002173 The aim of the present work was to compare various separation techniques for the fast analysis of closelyrelated compounds, including structurally-related compounds, positional isomers, diastereoisomers, Z/E isomers. Three analytical techniques were evaluated, namely ultra high performance liquid chromatography (UHPLC), ultra high performance supercritical fluid chromatography (UHPSFC), both with sub-2 µm particles, and capillary electrophoresis (CE) using non-aqueous solvents. To fairly compare the three analytical techniques, only two starting conditions for further method development were considered. All the selected mobile phases or background electrolyte were MS-compatible. As expected, CE often provided excellent results for the analysis of basic compounds but it was difficult to find out conditions that could be widely applied. On the other hand, UHPLC and UHPSFC were more generic and the performance was better than CE for the analysis of neutral and acidic compounds. In all cases, the analysis time was systematically lower than 3 min. In conclusion, UHPLC was the most versatile strategy for the analysis of closely-related compounds and should be tested in a first instance. UHPSFC and CE approaches offered some drastic changes in selectivity and should be considered a second choice to reach alternative selectivity as they also allow high throughput separations. Diastereoisomers Z/E isomers UHPLC Convergence chromatography NACE SFC Dewey 615 31 Section des sciences pharmaceutiques 17 false Journal of chromatography 1282 1afa7940-2108-4327-bac3-3ba946e3d93e Article (Accepted version) Restricted 2013 - structurally related compounds, J chrom A, 2013, 1282, 172.pdf 986750 application/pdf restrictedAccess Faculté des sciences / Section des sciences pharmaceutiques

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Evaluation and comparison of various separation techniques for the analysis of closely-related compounds of pharmaceutical interest	Journal of chromatography	2013	724	0
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Waller, Laura