Antimicrobial Peptide Dendrimers and Quorum-Sensing Inhibitors in Formulating Next-Generation Anti-Infection Cell Therapy Dressings for Burns

Article Article scientifique Article Antimicrobial Peptide Dendrimers and Quorum-Sensing Inhibitors in Formulating Next-Generation Anti-Infection Cell Therapy Dressings for Burns eng 2021-06-24 2021-06-24 author Jafari Paris 653732 author Luscher Alexandre 660541 author Siriwardena Thissa author Michetti Murielle author Que Yok-Ai author Rahme Laurence G author Reymond Jean-Louis 0000-0003-2724-2942 author Raffoul Wassim 0000-0002-3524-0077 author Van Delden Christian 24088 author Applegate Lee Ann 0000-0002-7346-9921 author Kohler Thilo 0000-0003-4889-9492 23327 3839 10.3390/molecules26133839 34202446 1420-3049 PMC8270311 https://www.mdpi.com/1420-3049/26/13/3839/htm Multidrug resistance infections are the main cause of failure in the pro-regenerative cell-mediated therapy of burn wounds. The collagen-based matrices for delivery of cells could be potential substrates to support bacterial growth and subsequent lysis of the collagen leading to a cell therapy loss. In this article, we report the development of a new generation of cell therapy formulations with the capacity to resist infections through the bactericidal effect of antimicrobial peptide dendrimers and the anti-virulence effect of anti-quorum sensing MvfR (PqsR) system compounds, which are incorporated into their formulation. Anti-quorum sensing compounds limit the pathogenicity and antibiotic tolerance of pathogenic bacteria involved in the burn wound infections, by inhibiting their virulence pathways. For the first time, we report a biological cell therapy dressing incorporating live progenitor cells, antimicrobial peptide dendrimers, and anti-MvfR compounds, which exhibit bactericidal and anti-virulence properties without compromising the viability of the progenitor cells. MvfR PqsR Anti-infection dressing Antimicrobial peptide dendrimers Biological bandage Burn wound Cell therapy Quorum-sensing inhibitors Anti-Bacterial Agents / chemistry Anti-Bacterial Agents / pharmacology Burns / microbiology Burns / pathology Burns / therapy Cell- and Tissue-Based Therapy Cells, Cultured Dendrimers / chemistry Dendrimers / pharmacology Humans Pore Forming Cytotoxic Proteins / chemistry Pore Forming Cytotoxic Proteins / pharmacology Pseudomonas Infections / drug therapy Pseudomonas Infections / metabolism Pseudomonas Infections / mortality Pseudomonas aeruginosa / growth & development Quorum Sensing / drug effects Dewey 616 Swiss National Science Foundation A Chemical Space Approach to Bioactive Peptides 200020_159941 CH Project funding SwissTransmed CRUS B5 platform 14/2013 56 Département de médecine 221 54 Département de microbiologie et médecine moléculaire 21B false 235 Quorom-sensing et gènes de virulence 301 Molecules 26 13 eccef138-6a68-42eb-a370-b8729ce889d9 Article (Published version) Public Article-scientifique-from-unpaywall.pdf 4107950 application/pdf CC BY-4.0 7944cfa1-5760-4168-9871-13e9c7aae8bc Appendix Public molecules-1227466-supplementary.pdf 383152 application/pdf Supplementary figures openAccess Faculté de médecine / Section de médecine clinique / Département de médecine Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire

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Antimicrobial Peptide Dendrimers and Quorum-Sensing Inhibitors in Formulating Next-Generation Anti-Infection Cell Therapy Dressings for Burns	Molecules			2021	129	166

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