The pseudoenzyme PDX1.2 boosts vitamin B6 biosynthesis under heat and oxidative stress in Arabidopsis

Article Article scientifique Article The pseudoenzyme PDX1.2 boosts vitamin B6 biosynthesis under heat and oxidative stress in Arabidopsis eng 2014 author Moccand Cyril 686582 author Boycheva Svetlana 685027 author Surriabre Dick Pedro Eduardo 645058 author Tambasco-Studart Marina author Raschke Maja author Kaufmann Markus 716671 author Fitzpatrick Thérésa Bridget 684438 8203-8216 10.1074/jbc.M113.540526 24505140 0021-9258 Vitamin B6 is an indispensable compound for survival, well known as a cofactor for numerous central metabolic enzymes and more recently for playing a role in several stress responses, particularly in association with oxidative stress. Regulatory aspects for the use of the vitamin in these roles are not known. Here we show that certain plants carry a pseudoenzyme (PDX1.2), which is involved in regulating vitamin B6 biosynthesis de novo under stress conditions. Specifically, we demonstrate that Arabidopsis PDX1.2 enhances the activity of its catalytic paralogs by forming a heterododecameric complex. PDX1.2 is strongly induced by heat as well as singlet oxygen stress, concomitant with an enhancement of vitamin B6 production. Analysis of pdx1.2 knockdown lines demonstrates that boosting vitamin B6 content is dependent on PDX1.2, revealing that this pseudoenzyme acts as a positive regulator of vitamin B6 biosynthesis during such stress conditions in plants. Amino Acid Sequence Arabidopsis/genetics/physiology Arabidopsis Proteins/chemistry/genetics/metabolism Gene Knockdown Techniques Hot Temperature Models Molecular Molecular Sequence Data Nitrogenous Group Transferases/chemistry/genetics/metabolism Oxidative Stress Stress Physiological Vitamin B 6/metabolism Dewey 580 45 Département des sciences végétales 163 false The Journal of biological chemistry 289 12 a7a6fb3a-5d94-429f-bf8c-ebf92966fb43 Article (Published version) Public Moccand.pdf 4178239 application/pdf openAccess Faculté des sciences / Section de biologie / Département des sciences végétales

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The pseudoenzyme PDX1.2 boosts vitamin B6 biosynthesis under heat and oxidative stress in Arabidopsis	The Journal of biological chemistry			2014	502	247

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Surriabre Dick, Pedro Eduardo