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Scientific article
Open access
English

The pseudoenzyme PDX1.2 boosts vitamin B6 biosynthesis under heat and oxidative stress in Arabidopsis

Published inThe Journal of biological chemistry, vol. 289, no. 12, p. 8203-8216
Publication date2014
Abstract

Vitamin B6 is an indispensable compound for survival, well known as a cofactor for numerous central metabolic enzymes and more recently for playing a role in several stress responses, particularly in association with oxidative stress. Regulatory aspects for the use of the vitamin in these roles are not known. Here we show that certain plants carry a pseudoenzyme (PDX1.2), which is involved in regulating vitamin B6 biosynthesis de novo under stress conditions. Specifically, we demonstrate that Arabidopsis PDX1.2 enhances the activity of its catalytic paralogs by forming a heterododecameric complex. PDX1.2 is strongly induced by heat as well as singlet oxygen stress, concomitant with an enhancement of vitamin B6 production. Analysis of pdx1.2 knockdown lines demonstrates that boosting vitamin B6 content is dependent on PDX1.2, revealing that this pseudoenzyme acts as a positive regulator of vitamin B6 biosynthesis during such stress conditions in plants.

Keywords
  • Amino Acid Sequence
  • Arabidopsis/genetics/physiology
  • Arabidopsis Proteins/chemistry/genetics/metabolism
  • Gene Knockdown Techniques
  • Hot Temperature
  • Models
  • Molecular
  • Molecular Sequence Data
  • Nitrogenous Group Transferases/chemistry/genetics/metabolism
  • Oxidative Stress
  • Stress
  • Physiological
  • Vitamin B 6/metabolism
Citation (ISO format)
MOCCAND, Cyril et al. The pseudoenzyme PDX1.2 boosts vitamin B6 biosynthesis under heat and oxidative stress in Arabidopsis. In: The Journal of biological chemistry, 2014, vol. 289, n° 12, p. 8203–8216. doi: 10.1074/jbc.M113.540526
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Article (Published version)
accessLevelPublic
Identifiers
ISSN of the journal0021-9258
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117downloads

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