Analysis of the intracellular localisation and alternatively spliced isoforms of splicing factor 1

Diplôme Thèse Analysis of the intracellular localisation and alternatively spliced isoforms of splicing factor 1 eng 2009-05-25 author Choleza Maria 635550 director Kraemer Angela 22808 10.13097/archive-ouverte/unige:5097 urn:nbn:ch:unige-50979 Sc. 4144 Splicing factor 1 is a protein with several isoforms produced by alternative splicing of a single premRNA. These isoforms share a common N-terminal half and have distinct C-terminal portions. In this study, isoforms of SF1 in different cell lines and tissues were identified, and their composition was investigated in different tissues. The results suggest that SF1 isoforms are expressed in a tissue-specific manner. The function of SF1 and its isoforms is not clear. To provide information towards that, we used fluorescence microscopy and demonstrated that SF1 is a new component of paraspeckles. Paraspeckles are formed around non-coding RNAs and although their function is not known, their protein content points to a role in transcriptional control and RNA metabolism. SF1 has been shown previously to bind to a number of ncRNAs. Results from this thesis support the above finding and show that ncRNAs present in paraspeckles associate with SF1. Splicing factor 1 Paraspeckles Speckles Isoforms Dewey 570 46 Département de biologie cellulaire 165 false b87aeedf-c42f-4d9d-a618-49d4ec1dd5ce Thesis Public MariaCholeza_Thesis.pdf 7733023 application/pdf Restricted 2010-12-03 f53deeba87e93b469c9337d40c392e8921afa14670607682042532648919014c openAccess Faculté des sciences / Section de biologie / Département de biologie cellulaire

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Analysis of the intracellular localisation and alternatively spliced isoforms of splicing factor 1				2009	850	411

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Choleza, Maria