UNIGE document Doctoral Thesis
previous document  unige:5097  next document
add to browser collection
Title

Analysis of the intracellular localisation and alternatively spliced isoforms of splicing factor 1

Author
Director
Defense Thèse de doctorat : Univ. Genève, 2009 - Sc. 4144 - 2009/05/25
Abstract Splicing factor 1 is a protein with several isoforms produced by alternative splicing of a single premRNA. These isoforms share a common N-terminal half and have distinct C-terminal portions. In this study, isoforms of SF1 in different cell lines and tissues were identified, and their composition was investigated in different tissues. The results suggest that SF1 isoforms are expressed in a tissue-specific manner. The function of SF1 and its isoforms is not clear. To provide information towards that, we used fluorescence microscopy and demonstrated that SF1 is a new component of paraspeckles. Paraspeckles are formed around non-coding RNAs and although their function is not known, their protein content points to a role in transcriptional control and RNA metabolism. SF1 has been shown previously to bind to a number of ncRNAs. Results from this thesis support the above finding and show that ncRNAs present in paraspeckles associate with SF1.
Keywords Splicing factor 1ParaspecklesSpecklesIsoforms
Identifiers
URN: urn:nbn:ch:unige-50979
Full text
Thesis (7.4 MB) - public document Free access
Structures
Citation
(ISO format)
CHOLEZA, Maria. Analysis of the intracellular localisation and alternatively spliced isoforms of splicing factor 1. Université de Genève. Thèse, 2009. https://archive-ouverte.unige.ch/unige:5097

310 hits

246 downloads

Update

Deposited on : 2010-02-01

Export document
Format :
Citation style :