Pseudomonas aeruginosa rhamnolipid micelles deliver toxic metabolites and antibiotics into Staphylococcus aureus

Article Article scientifique Article Pseudomonas aeruginosa rhamnolipid micelles deliver toxic metabolites and antibiotics into Staphylococcus aureus eng 2021-12-20 2022-01-21 author Gdaniec Bartosz Gérard 860784 author Bonini Fabien 863519 author Prodon Charles François 56818 author Braschler Thomas Michaël 848483 author Kohler Thilo 0000-0003-4889-9492 23327 author Van Delden Christian 24088 103669 10.1016/j.isci.2021.103669 35028539 2589-0042 PMC8741607 https://www.sciencedirect.com/science/article/pii/S2589004221016394 Efficient delivery of toxic compounds to bacterial competitors is essential during interspecies microbial warfare. Rhamnolipids (RLPs) are glycolipids produced by Pseudomonas and Burkholderia species involved in solubilization and uptake of environmental aliphatic hydrocarbons and perform as biosurfactants for swarming motility. Here, we show that RLPs produced by Pseudomonas aeruginosa associate to form micelles. Using high-resolution microscopy, we found that RLP micelles serve as carriers for self-produced toxic compounds, which they deliver to Staphylococcus aureus cells, thereby enhancing and accelerating S. aureus killing. RLPs also potentiated the activity of lincosamide antibiotics, suggesting that RLP micelles may transport not only self-produced but also heterologous compounds to target competing bacterial species. Lipid Microbiology Dewey 616 Dewey 616.07 Fondation Bertarelli Swiss National Science Foundation Pseudomonas aeruginosa in lung-transplant recipients: exploiting adaptation and microbial interactions to fight bacterial infections 179289 CH Project funding Swiss National Science Foundation Brain Patch Technology 163684 CH Careers Swiss National Science Foundation Cell Patch Technology 194813 CH Careers 56 Département de médecine 221 54 Département de microbiologie et médecine moléculaire 21B 137 Département de pathologie et immunologie 21F false 972 Groupe Thomas Braschler 970 375 KOHLER THILO 641 235 Perception du Quorum et gènes de virulence 301 https://doi.org/10.5281/zenodo.5159447 iScience 25 1 05c06752-a04b-4c3d-9928-581028455642 Article (Published version) Public Article-scientifique-from-europePmc.pdf 3863584 application/pdf CC BY-NC-ND-4.0 7d125c4f-8245-4975-9443-7f62f7ac5529 Appendix Public mmc1.pdf 417083 application/pdf Figures S1–S3 and Table S1 Vignette Public 1-s2.0-S2589004221016394-fx1_lrg.jpg 226333 image/jpeg openAccess Faculté de médecine / Section de médecine clinique / Département de médecine Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie

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Pseudomonas aeruginosa rhamnolipid micelles deliver toxic metabolites and antibiotics into Staphylococcus aureus	iScience			2022	266	126

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Prodon, Charles François