Structural basis for the molecular recognition between human splicing factors U2AF65 and SF1/mBBP

Article Article scientifique Article Structural basis for the molecular recognition between human splicing factors U2AF65 and SF1/mBBP eng 2003 author Selenko Philipp author Gregorovic Goran 531348 author Sprangers Remco author Stier Gunter author Rhani Zakaria 93937 author Kraemer Angela 22808 author Sattler Michael 965-976 10.1016/S1097-2765(03)00115-1 12718882 1097-2765 http://preview.ncbi.nlm.nih.gov/pubmed/12718882 The essential splicing factors SF1 and U2AF play an important role in the recognition of the pre-mRNA 3' splice site during early spliceosome assembly. The structure of the C-terminal RRM (RRM3) of human U2AF(65) complexed to an N-terminal peptide of SF1 reveals an extended negatively charged helix A and an additional helix C. Helix C shields the potential RNA binding surface. SF1 binds to the opposite, helical face of RRM3. It inserts a conserved tryptophan into a hydrophobic pocket between helices A and B in a way that strikingly resembles part of the molecular interface in the U2AF heterodimer. This molecular recognition establishes a paradigm for protein binding by a subfamily of noncanonical RRMs. Collboration between Krämer and Sattler groups Amino Acid Sequence/genetics Binding Sites/genetics DNA Mutational Analysis DNA-Binding Proteins Humans Molecular Conformation Molecular Structure Nuclear Proteins Protein Structure, Secondary/genetics Protein Structure, Tertiary/genetics RNA Splice Sites/genetics RNA Splicing/genetics RNA, Messenger/genetics/metabolism RNA-Binding Proteins/genetics/metabolism Ribonucleoproteins/genetics/metabolism Sequence Homology, Amino Acid Transcription Factors Dewey 570 46 Département de biologie cellulaire 165 false Molecular cell 11 4 Faculté des sciences / Section de biologie / Département de biologie cellulaire

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Structural basis for the molecular recognition between human splicing factors U2AF65 and SF1/mBBP	Molecular cell			2003	586	0

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Gregorovic, Goran