UNIGE document Scientific Article
previous document  unige:17544  next document
add to browser collection
Title

Structural basis for the molecular recognition between human splicing factors U2AF65 and SF1/mBBP

Authors
Selenko, Philipp
Sprangers, Remco
Stier, Gunter
Sattler, Michael
Published in Molecular Cell. 2003, vol. 11, no. 4, p. 965-76
Abstract The essential splicing factors SF1 and U2AF play an important role in the recognition of the pre-mRNA 3' splice site during early spliceosome assembly. The structure of the C-terminal RRM (RRM3) of human U2AF(65) complexed to an N-terminal peptide of SF1 reveals an extended negatively charged helix A and an additional helix C. Helix C shields the potential RNA binding surface. SF1 binds to the opposite, helical face of RRM3. It inserts a conserved tryptophan into a hydrophobic pocket between helices A and B in a way that strikingly resembles part of the molecular interface in the U2AF heterodimer. This molecular recognition establishes a paradigm for protein binding by a subfamily of noncanonical RRMs.
Keywords Amino Acid Sequence/geneticsBinding Sites/geneticsDNA Mutational AnalysisDNA-Binding ProteinsHumansMolecular ConformationMolecular StructureNuclear ProteinsProtein Structure, Secondary/geneticsProtein Structure, Tertiary/geneticsRNA Splice Sites/geneticsRNA Splicing/geneticsRNA, Messenger/genetics/metabolismRNA-Binding Proteins/genetics/metabolismRibonucleoproteins/genetics/metabolismSequence Homology, Amino AcidTranscription Factors
Identifiers
PMID: 12718882
Note Collboration between Krämer and Sattler groups
Full text
Structures
Citation
(ISO format)
SELENKO, Philipp et al. Structural basis for the molecular recognition between human splicing factors U2AF65 and SF1/mBBP. In: Molecular Cell, 2003, vol. 11, n° 4, p. 965-76. https://archive-ouverte.unige.ch/unige:17544

121 hits

0 download

Update

Deposited on : 2011-11-25

Export document
Format :
Citation style :