Sendai virus induced cytoplasmic actin remodeling correlates with efficient virus particle production

Article Article scientifique Article Sendai virus induced cytoplasmic actin remodeling correlates with efficient virus particle production eng 2011 author Miazza Vincent Xavier 510406 author Mottet-Osman Geneviève 26297 author Startchik Sergei 27116 author Chaponnier Christine 25579 author Roux Laurent 24751 7-16 10.1016/j.virol.2010.10.003 21075412 0042-6822 Cytoplasmic actins have been found interacting with viral proteins and identified in virus particles. We analyzed by confocal microscopy the cytoplasmic β- and γ-actin patterns during the course of Sendai virus infections in polarized cells. We observed a spectacular remodeling of the β-cytoplasmic actin which correlated with productive viral multiplication. Conversely, suppression of M during the course of a productive infection resulted in the decrease of particle production and the absence of β-actin remodeling. As concomitant suppression of β- and γ-actins resulted as well in reduction of virus particle production, we propose that Sendai virus specifically induces actin remodeling in order to promote efficient virion production. Beta- and γ-cytoplasmic actin recruitment could substitute for that of the endosomal sorting complex required for transport (ESCRT) mobilized by other enveloped viruses but apparently not used by Sendai virus. Actins/metabolism Animals Cell Line Cytoplasm/metabolism Dogs Gene Expression Regulation, Viral/physiology Microscopy, Confocal Protein Isoforms RNA, Small Interfering Sendai virus/physiology Viral Matrix Proteins/genetics/metabolism Virion/physiology Virus Replication/physiology Dewey 616 Dewey 616.07 Swiss National Science Foundation Le cycle de multiplication d'un Paramyxovirus: assemblage des différents constituants et production de particules virales 122462 CH Project funding Swiss National Science Foundation Morphogenesis and production of a Paramyxovirus particles 138465 CH Project funding 54 Département de microbiologie et médecine moléculaire 21B 137 Département de pathologie et immunologie 21F false 140 Groupe Christine Chaponnier (pathologie et immunologie) 171 224 Multiplication virale 286 Virology 410 1 fdbf042c-6561-42d3-b350-d0ba27df4c84 Article (Published version) Public Miazza-Roux_virology 2011.pdf 2558467 application/pdf openAccess Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie

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Sendai virus induced cytoplasmic actin remodeling correlates with efficient virus particle production	Virology	2011	742	453

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Miazza, Vincent Xavier