An adhesion molecule in free-living Dictyostelium amoebae with integrin beta features

Article Article scientifique Article An adhesion molecule in free-living Dictyostelium amoebae with integrin beta features eng 2006 author Cornillon Sophie Marie 508815 author Gebbie Leigh 528871 author Benghezal Mohammed 133426 author Nair Prashant 629600 author Keller Sébastien 502454 author Wehrle-Haller Bernhard 0000-0002-1159-1147 116298 author Charette Steve J author Brückert Franz author Letourneur François author Cosson Pierre 0000-0002-4881-0358 119596 617-621 10.1038/sj.embor.7400701 16699495 1469-221X The study of free-living amoebae has proven valuable to explain the molecular mechanisms controlling phagocytosis, cell adhesion and motility. In this study, we identified a new adhesion molecule in Dictyostelium amoebae. The SibA (Similar to Integrin Beta) protein is a type I transmembrane protein, and its cytosolic, transmembrane and extracellular domains contain features also found in integrin beta chains. In addition, the conserved cytosolic domain of SibA interacts with talin, a well-characterized partner of mammalian integrins. Finally, genetic inactivation of SIBA affects adhesion to phagocytic particles, as well as cell adhesion and spreading on its substrate. It does not visibly alter the organization of the actin cytoskeleton, cellular migration or multicellular development. Our results indicate that the SibA protein is a Dictyostelium cell adhesion molecule presenting structural and functional similarities to metazoan integrin beta chains. This study sheds light on the molecular mechanisms controlling cell adhesion and their establishment during evolution. Amino Acid Motifs Amino Acid Sequence Animals Cell Adhesion Cell Adhesion Molecules/chemistry/genetics/metabolism Cell Movement Conserved Sequence Cytosol/metabolism Dictyostelium/genetics Genes Protozoan Glutathione Transferase/metabolism Integrins/chemistry/genetics/metabolism Molecular Sequence Data Mutation Protein Sorting Signals Protein Structure Tertiary Recombinant Fusion Proteins/chemistry/metabolism Recombination Genetic Talin/metabolism Dewey 612 134 Département de physiologie cellulaire et métabolisme 21C false 379 Migration cellulaire 645 111 Phagocytose 140 EMBO reports 7 6 e7cc2ebe-ccc1-4d6a-932a-1bea32d6706c Article (Published version) Public Cornillion EMBO rep 2006.pdf 189183 application/pdf openAccess Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme

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An adhesion molecule in free-living Dictyostelium amoebae with integrin beta features	EMBO reports			2006	659	267

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Keller, Sébastien