An adhesion molecule in free-living Dictyostelium amoebae with integrin beta features

Cornillon, Sophie Marie; Gebbie, Leigh; Benghezal, Mohammed; Nair, Prashant; Keller, Sébastien; Wehrle-Haller, Bernhard; Charette, Steve J; Brückert, Franz; Letourneur, François; Cosson, Pierre

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Title		An adhesion molecule in free-living Dictyostelium amoebae with integrin beta features
Authors		Cornillon, Sophie Marie Gebbie, Leigh Benghezal, Mohammed Nair, Prashant Keller, Sébastien Wehrle-Haller, Bernhard Charette, Steve J Brückert, Franz Letourneur, François Cosson, Pierre show all authors [1 - 10]
Published in		EMBO Reports. 2006, vol. 7, no. 6, p. 617-21
Abstract		The study of free-living amoebae has proven valuable to explain the molecular mechanisms controlling phagocytosis, cell adhesion and motility. In this study, we identified a new adhesion molecule in Dictyostelium amoebae. The SibA (Similar to Integrin Beta) protein is a type I transmembrane protein, and its cytosolic, transmembrane and extracellular domains contain features also found in integrin beta chains. In addition, the conserved cytosolic domain of SibA interacts with talin, a well-characterized partner of mammalian integrins. Finally, genetic inactivation of SIBA affects adhesion to phagocytic particles, as well as cell adhesion and spreading on its substrate. It does not visibly alter the organization of the actin cytoskeleton, cellular migration or multicellular development. Our results indicate that the SibA protein is a Dictyostelium cell adhesion molecule presenting structural and functional similarities to metazoan integrin beta chains. This study sheds light on the molecular mechanisms controlling cell adhesion and their establishment during evolution.
Keywords		Amino Acid Motifs — Amino Acid Sequence — Animals — Cell Adhesion — Cell Adhesion Molecules/chemistry/genetics/metabolism — Cell Movement — Conserved Sequence — Cytosol/metabolism — Dictyostelium/genetics — Genes — Protozoan — Glutathione Transferase/metabolism — Integrins/chemistry/genetics/metabolism — Molecular Sequence Data — Mutation — Protein Sorting Signals — Protein Structure — Tertiary — Recombinant Fusion Proteins/chemistry/metabolism — Recombination — Genetic — Talin/metabolism
Identifiers		DOI: 10.1038/sj.embor.7400701 PMID: 16699495
Full text		Article (Published version) (185 Kb) - Free access
Structures		Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme
Research groups		Phagocytose (140) Migration cellulaire (645)
Citation (ISO format)		CORNILLON, Sophie Marie et al. An adhesion molecule in free-living Dictyostelium amoebae with integrin beta features. In: EMBO Reports, 2006, vol. 7, n° 6, p. 617-21. https://archive-ouverte.unige.ch/unige:26027