Identification of a measles variant displaying mutations impacting molecular diagnostics, Geneva, Switzerland, 2023

Article Article scientifique Article Identification of a measles variant displaying mutations impacting molecular diagnostics, Geneva, Switzerland, 2023 eng 2024-02 author Perez Rodriguez Francisco Javier Geneva Center for Emerging Viral Diseases, Geneva University Hospitals, Geneva, Switzerland.,National Measles and Rubella Reference Laboratory (CNRRR), Geneva University Hospitals, Geneva, Switzerland.,Laboratory of virology, Laboratory Medicine Division, Geneva University Hospitals, Geneva, Switzerland. 868142 author Cherpillod Pascal Geneva Center for Emerging Viral Diseases, Geneva University Hospitals, Geneva, Switzerland.,National Measles and Rubella Reference Laboratory (CNRRR), Geneva University Hospitals, Geneva, Switzerland.,Laboratory of virology, Laboratory Medicine Division, Geneva University Hospitals, Geneva, Switzerland. 650028 author Thomasson Valentine Laboratory of virology, Laboratory Medicine Division, Geneva University Hospitals, Geneva, Switzerland. 417481 author Vetter Pauline 0000-0002-9902-5069 Geneva Center for Emerging Viral Diseases, Geneva University Hospitals, Geneva, Switzerland.,Laboratory of virology, Laboratory Medicine Division, Geneva University Hospitals, Geneva, Switzerland. 673142 author Schibler Manuel Geneva Center for Emerging Viral Diseases, Geneva University Hospitals, Geneva, Switzerland.,National Measles and Rubella Reference Laboratory (CNRRR), Geneva University Hospitals, Geneva, Switzerland.,Laboratory of virology, Laboratory Medicine Division, Geneva University Hospitals, Geneva, Switzerland. 96444 Département de médecine (DEMED) 2400034 10.2807/1560-7917.ES.2024.29.5.2400034 38304951 1025-496X PMC10835752 https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2024.29.5.2400034 Real-time PCR is one of the most widely used techniques to diagnose measles cases. Here we report measles virus variants with three genetic mutations in the reverse primer annealing site of a widely used PCR. The mutations result in a slight loss of the PCR sensitivity. Variants bearing the three mutations presently circulate in different countries since at least the end of 2021. Our findings highlight the usefulness of molecular surveillance in monitoring if oligonucleotides in diagnostic tests remain adequate. PCR primer mismatch PCR sensitivity Genetic variability Measles Humans Switzerland Pathology, Molecular RNA, Viral / genetics Measles / diagnosis Measles / epidemiology Measles virus / genetics Mutation Sensitivity and Specificity Reverse Transcriptase Polymerase Chain Reaction Dewey 616 56 Département de médecine 221 false 1255 Virus émergents 993 400 Virologie clinique 668 526 Résistance aux antibiotiques 866 235 Perception du Quorum et gènes de virulence 301 https://www.ncbi.nlm.nih.gov/nuccore/PP229487 https://www.ncbi.nlm.nih.gov/nuccore/PP229488 https://www.ncbi.nlm.nih.gov/nuccore/PP229489 https://www.ncbi.nlm.nih.gov/nuccore/PP229490 https://www.ncbi.nlm.nih.gov/nuccore/PP229491 https://www.ncbi.nlm.nih.gov/nuccore/PP229492 https://www.ncbi.nlm.nih.gov/nuccore/PP229493 https://www.ncbi.nlm.nih.gov/nuccore/PP229494 https://www.ncbi.nlm.nih.gov/nuccore/PP229495.1/ Euro surveillance 29 5 ad3f2db3-9357-4bc4-ac27-cc61df6e70a5 Article (Published version) Public Article_scientifique_from_europePmc.pdf 192751 application/pdf CC BY-4.0 4a677cedcf0aa3437a307516656575632455b41748287e0abc5252ea41606cd7 openAccess Faculté de médecine / Section de médecine clinique / Département de médecine

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Identification of a measles variant displaying mutations impacting molecular diagnostics, Geneva, Switzerland, 2023	Euro surveillance			2024	80	168

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