Ca2+ and Mn2+ mediated binding of the glycoprotein peroxidase to membranes of Pharbitis cotyledons

Article Article scientifique Article Ca2+ and Mn2+ mediated binding of the glycoprotein peroxidase to membranes of Pharbitis cotyledons eng 1985 author Kiefer Stefanie Marta 32107 author Penel Claude 24695 author Greppin Hubert 24907 37-43 10.1016/0168-9452(85)90189-X 0168-9452 http://linkinghub.elsevier.com/retrieve/pii/016894528590189X Ca²⁺ and Mn²⁺ promote the binding of the basic isoperoxidase to a crude membrane preparation in extracts from Pharbitis cotyledons. The Ca²⁺- or Mn²⁺-induced binding is resistant to high ionic strength and can be saturated by increasing the divalent ion or the isoperoxidase concentrations. Treatments in vitro with glucosaminidase or in vivo with tunicamycin show that the carbohydrate part of the isoperoxidase is necessary for the binding. The amino sugar galactosamine inhibits the binding at rather high concentrations. Pharbitis basic isoperoxidase can be bound to zucchini squash microsomes in the presence of Ca²⁺ and conversely. Dewey 580 45 Département des sciences végétales 163 false Plant Science 39 1 8fe90a31-8667-4f3c-ac5d-69d3df30ceaf Article (Published version) Restricted 1-s2.0-016894528590189X-main.pdf 452254 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département des sciences végétales

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Ca²⁺ and Mn²⁺ mediated binding of the glycoprotein peroxidase to membranes of Pharbitis cotyledons	Plant Science			1985	404	0

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Kiefer, Stefanie Marta