Scientific article

Ca2+ and Mn2+ mediated binding of the glycoprotein peroxidase to membranes of Pharbitis cotyledons

Published inPlant Science, vol. 39, no. 1, p. 37-43
Publication date1985

Ca²⁺ and Mn²⁺ promote the binding of the basic isoperoxidase to a crude membrane preparation in extracts from Pharbitis cotyledons. The Ca²⁺- or Mn²⁺-induced binding is resistant to high ionic strength and can be saturated by increasing the divalent ion or the isoperoxidase concentrations. Treatments in vitro with glucosaminidase or in vivo with tunicamycin show that the carbohydrate part of the isoperoxidase is necessary for the binding. The amino sugar galactosamine inhibits the binding at rather high concentrations. Pharbitis basic isoperoxidase can be bound to zucchini squash microsomes in the presence of Ca²⁺ and conversely.

Citation (ISO format)
KIEFER, Stefanie Marta, PENEL, Claude, GREPPIN, Hubert. Ca<sup>2+</sup> and Mn<sup>2+</sup> mediated binding of the glycoprotein peroxidase to membranes of <i>Pharbitis</i> cotyledons. In: Plant Science, 1985, vol. 39, n° 1, p. 37–43. doi: 10.1016/0168-9452(85)90189-X
Main files (1)
Article (Published version)
ISSN of the journal0168-9452

Technical informations

Creation01/23/2019 11:07:00 AM
First validation01/23/2019 11:07:00 AM
Update time03/15/2023 3:37:49 PM
Status update03/15/2023 3:37:49 PM
Last indexation01/17/2024 4:45:34 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack