Fibronectin Gene Expression in Proliferating, Quiescent, and SV40-Infected Mouse Kidney Cells

Article Article scientifique Article Fibronectin Gene Expression in Proliferating, Quiescent, and SV40-Infected Mouse Kidney Cells eng 1992 author Khandjian Edouard W. author Salomon Maria Consuelo 25505 author Leonard Nicole 25806 author Tremblay Sandra author Turler Hans 25123 464-470 10.1016/0014-4827(92)90100-M 0014-4827 https://linkinghub.elsevier.com/retrieve/pii/001448279290100M To study alterations in cellular gene expression in mouse kidney cell cultures infected with simian virus 40 (SV40) or polyomavirus, we performed a differential screening of a mouse kidney cDNA library with probes prepared from mRNAs of virus-infected and mock-infected cells. We isolated and characterized cDNA recombinant pKT13 which detected increased mRNA levels in infected cells. Sequence analysis of pKT13 revealed close to 100% homology with the 3′-end of mouse fibronectin (FN) mRNA. Since primary cultures of baby mouse kidney cells have been extensively characterized in our laboratories, we studied FN gene expression at different stages of uninfected and virus-infected cultures. High levels of FN and of its mRNA were found in the kidneys of suckling mice, while in primary cultures of proliferating epithelial kidney cells the expression of FN was very low until the cultures became confluent. Thereafter FN increased and reached high levels in cells which were irreversibly arrested in phase G0 and which had apparently exhausted their finite division potential. Infection of confluent cultures with polyomavirus or SV40 resulted in a further stimulation of FN gene expression. However, during abortive infection with SV40, FN mRNA and FN levels decreased with emergence of transformed cells and were low in an established SV40-transformed mouse kidney cell line. These changes in FN gene expression suggest that high levels of FN might be indicative in vivo for terminal differentiation and in vitro for cellular senescence. Dewey 570 Swiss National Science Foundation 3.403.086 CH Swiss National Science Foundation 31-26332.89 CH 43 Département de biologie moléculaire 161 false Experimental Cell Research 202 2 6e2e15a5-cb98-441c-b277-114df9ee898f Article (Published version) Restricted 1-s2.0-001448279290100M-main.pdf 2547944 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire

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Fibronectin Gene Expression in Proliferating, Quiescent, and SV40-Infected Mouse Kidney Cells	Experimental Cell Research			1992	248	0

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Leonard, Nicole