Presence of a truncated form of the Sendai virus P protein in a long-term persistent infection: implications for the maintenance of the persistent state

Article Article scientifique Article Presence of a truncated form of the Sendai virus P protein in a long-term persistent infection: implications for the maintenance of the persistent state eng 1994 author Garcin Dominique 24296 author De Melo Curran Merivane 22958 author Roux Laurent 24751 author Kolakofsky Daniel 25420 author Curran Joseph 22465 19-25 10.1006/viro.1994.1261 8178486 0042-6822 In this report we have monitored viral gene expression, both at the RNA and protein level, after the establishment of a long-term persistent infection of Sendai virus. The persistent infection was initially established by infecting BHK cells with a viral stock containing a short (1.4 kb) copy-back DI (DIH4). After over 120 weeks in culture this short copy-back DI had been replaced by two large deletion DIs (approximately 7 and 12 kb) from which was expressed an N-terminally truncated form of the P protein. The mRNA for this protein was detected in cells and the deletion within the P gene was mapped by PCR cloning and sequencing of intracellular nucleocapsid RNA. This truncated P protein (derived by deleting the N-terminal half of the cloned Pwt gene) has already been shown to function as a dominant negative for DI replication when driven by cloned viral genes. Cloning and expression of the truncated P from the long-term persistent infection revealed that this protein had retained the dominant negative phenotype. The presence of such a protein would severely depress viral gene expression and may therefore play an important role in the maintenance of persistence. Animals Base Sequence Cell Line Cloning, Molecular Cricetinae Defective Viruses/physiology Gene Expression Regulation, Viral/genetics Genes, Dominant Genes, Viral/genetics Molecular Sequence Data Parainfluenza Virus 1, Human/physiology Phosphoproteins/analysis/genetics/physiology RNA, Viral/analysis Viral Proteins/analysis/genetics/physiology Dewey 616 54 Département de microbiologie et médecine moléculaire 21B false 365 Régulation traductionnelle dans les cellules de mammifère 631 216 Virologie moléculaire 275 Virology 201 1 5613b73e-42ff-4363-a58d-e49b90beb9b1 Article (Published version) Restricted 1-s2.0-S004268228471261X-main.pdf 557365 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire

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Presence of a truncated form of the Sendai virus P protein in a long-term persistent infection: implications for the maintenance of the persistent state	Virology			1994	589	0

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De Melo Curran, Merivane