UNIGE document Scientific Article
previous document  unige:37931  next document
add to browser collection
Title

Presence of a truncated form of the Sendai virus P protein in a long-term persistent infection: implications for the maintenance of the persistent state

Authors
Published in Virology. 1994, vol. 201, no. 1, p. 19-25
Abstract In this report we have monitored viral gene expression, both at the RNA and protein level, after the establishment of a long-term persistent infection of Sendai virus. The persistent infection was initially established by infecting BHK cells with a viral stock containing a short (1.4 kb) copy-back DI (DIH4). After over 120 weeks in culture this short copy-back DI had been replaced by two large deletion DIs (approximately 7 and 12 kb) from which was expressed an N-terminally truncated form of the P protein. The mRNA for this protein was detected in cells and the deletion within the P gene was mapped by PCR cloning and sequencing of intracellular nucleocapsid RNA. This truncated P protein (derived by deleting the N-terminal half of the cloned Pwt gene) has already been shown to function as a dominant negative for DI replication when driven by cloned viral genes. Cloning and expression of the truncated P from the long-term persistent infection revealed that this protein had retained the dominant negative phenotype. The presence of such a protein would severely depress viral gene expression and may therefore play an important role in the maintenance of persistence.
Keywords AnimalsBase SequenceCell LineCloning, MolecularCricetinaeDefective Viruses/physiologyGene Expression Regulation, Viral/geneticsGenes, DominantGenes, Viral/geneticsMolecular Sequence DataParainfluenza Virus 1, Human/physiologyPhosphoproteins/analysis/genetics/physiologyRNA, Viral/analysisViral Proteins/analysis/genetics/physiology
Identifiers
PMID: 8178486
Full text
Article (Published version) (544 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research groups Virologie moléculaire (275)
Régulation traductionnelle dans les cellules de mammifère (631)
Citation
(ISO format)
GARCIN, Dominique et al. Presence of a truncated form of the Sendai virus P protein in a long-term persistent infection: implications for the maintenance of the persistent state. In: Virology, 1994, vol. 201, n° 1, p. 19-25. https://archive-ouverte.unige.ch/unige:37931

236 hits

0 download

Update

Deposited on : 2014-06-18

Export document
Format :
Citation style :