en
Scientific article
English

Presence of a truncated form of the Sendai virus P protein in a long-term persistent infection: implications for the maintenance of the persistent state

Published inVirology, vol. 201, no. 1, p. 19-25
Publication date1994
Abstract

In this report we have monitored viral gene expression, both at the RNA and protein level, after the establishment of a long-term persistent infection of Sendai virus. The persistent infection was initially established by infecting BHK cells with a viral stock containing a short (1.4 kb) copy-back DI (DIH4). After over 120 weeks in culture this short copy-back DI had been replaced by two large deletion DIs (approximately 7 and 12 kb) from which was expressed an N-terminally truncated form of the P protein. The mRNA for this protein was detected in cells and the deletion within the P gene was mapped by PCR cloning and sequencing of intracellular nucleocapsid RNA. This truncated P protein (derived by deleting the N-terminal half of the cloned Pwt gene) has already been shown to function as a dominant negative for DI replication when driven by cloned viral genes. Cloning and expression of the truncated P from the long-term persistent infection revealed that this protein had retained the dominant negative phenotype. The presence of such a protein would severely depress viral gene expression and may therefore play an important role in the maintenance of persistence.

Keywords
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • Cricetinae
  • Defective Viruses/physiology
  • Gene Expression Regulation, Viral/genetics
  • Genes, Dominant
  • Genes, Viral/genetics
  • Molecular Sequence Data
  • Parainfluenza Virus 1, Human/physiology
  • Phosphoproteins/analysis/genetics/physiology
  • RNA, Viral/analysis
  • Viral Proteins/analysis/genetics/physiology
Citation (ISO format)
GARCIN, Dominique et al. Presence of a truncated form of the Sendai virus P protein in a long-term persistent infection: implications for the maintenance of the persistent state. In: Virology, 1994, vol. 201, n° 1, p. 19–25. doi: 10.1006/viro.1994.1261
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0042-6822
565views
0downloads

Technical informations

Creation16.06.2014 14:11:00
First validation16.06.2014 14:11:00
Update time14.03.2023 21:21:59
Status update14.03.2023 21:21:59
Last indexation16.01.2024 11:05:15
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack