Comparison of the lipid composition of porcine buccal and esophageal permeability barriers

Article Article scientifique Article Comparison of the lipid composition of porcine buccal and esophageal permeability barriers eng 2005 author Diaz Isabel 134288 author Jacques Yves author Pizzolato Gian-Paolo author Guy Richard H. 109747 author Falson Françoise 981-987 10.1016/j.archoralbio.2005.04.008 15955526 0003-9969 Pig esophageal mucosa has been shown to be a useful and practical substitute for buccal mucosa in in vitro permeability studies in that it offers a larger surface area and it is much easier to prepare. Further, the tissues demonstrate similar histological characteristics. The objectives of this work were to characterize the lipid composition of the esophageal mucosa, to compare it to that of the buccal tissue, and to correlate lipid composition with the membranes' permeability to fentanyl. The major lipid classes of buccal and esophageal epithelia were separated and analysed by automated multiple development high-performance thin-layer chromatography (AMD-HPTLC). The two epithelia presented a very similar lipid pattern. In general, there were more polar lipids than non-polar; glycosylceramides were relatively abundant whereas the amount of ceramides present was very small. The flux of fentanyl applied as the citrate in aqueous solution was comparable across the buccal and esophageal barriers. Lipid extraction provoked a significant increase in permeability. In conclusion, this research confirms the suitability of the esophageal mucosa as a model for buccal permeability studies. Esophageal epithelium Buccal epithelium Membrane lipids Permeability AMD-HPTLC Dewey 615 31 Section des sciences pharmaceutiques 17 false Archives of oral biology 50 12 50907ffe-15ab-4054-920b-c36932f13011 Article (Published version) Restricted I. Diaz-del Consuelo.pdf 301583 application/pdf restrictedAccess Faculté des sciences / Section des sciences pharmaceutiques

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Comparison of the lipid composition of porcine buccal and esophageal permeability barriers	Archives of oral biology			2005	490	0

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Diaz, Isabel