Role of LBPA and Alix in Multivesicular Liposome Formation and Endosome Organization

Article Article scientifique Article Role of LBPA and Alix in Multivesicular Liposome Formation and Endosome Organization eng 2004 author Matsuo Hirotami 133371 author Chevallier Julien 125250 author Mayran Nathalie 507602 author Le Blanc Isabelle 532769 author Ferguson Charles author Faure Julien 514837 author Satori Blanc Nathalie author Matile Stefan 0000-0002-8537-8349 129654 author Dubochet Jacques author Sadoul Rémy author Parton Robert G. author Vilbois Francis author Gruenberg Jean 29661 531-534 10.1126/science.1092425 14739459 0036-8075 What are the components that control the assembly of subcellular organelles in eukaryotic cells? Although membranes can clearly be distorted by cytosolic factors, very little is known about the intrinsic mechanisms that control the biogenesis, shape, and organization of organellar membranes. Here, we found that the unconventional phospholipid lysobisphosphatidic acid (LBPA) could induce the formation of multivesicular liposomes that resembled the multivesicular endosomes that exist where this lipid is found in vivo. This process depended on the same pH gradient that exists across endosome membranes in vivo and was selectively controlled by Alix. In turn, Alix regulated the organization of LBPA-containing endosomes in vivo. Dewey 540 36 Département de chimie organique 141 false Science 303 5657 ae4ba178-bd9d-494f-b6ad-d0a27991b6c2 Article Restricted file1.pdf 477616 application/pdf restrictedAccess Faculté des sciences / Section de chimie et biochimie / Département de chimie organique

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Role of LBPA and Alix in Multivesicular Liposome Formation and Endosome Organization	Science			2004	788	0

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Matsuo, Hirotami