UNIGE document Scientific Article
previous document  unige:98893  next document
add to browser collection
Title

Lipidomics reveals diurnal lipid oscillations in human skeletal muscle persisting in cellular myotubes cultured in vitro

Authors
Betts, James A
Walhin, Jean-Philippe
Templeman, Iain
Chanon, Stéphanie
Weger, Benjamin D
show hidden authors show all authors [1 - 18]
Published in Proceedings of the National Academy of Sciences. 2017, vol. 114, no. 41, p. E8565-E8574
Abstract Circadian clocks play an important role in lipid homeostasis, with impact on various metabolic diseases. Due to the central role of skeletal muscle in whole-body metabolism, we aimed at studying muscle lipid profiles in a temporal manner. Moreover, it has not been shown whether lipid oscillations in peripheral tissues are driven by diurnal cycles of rest-activity and food intake or are able to persist in vitro in a cell-autonomous manner. To address this, we investigated lipid profiles over 24 h in human skeletal muscle in vivo and in primary human myotubes cultured in vitro. Glycerolipids, glycerophospholipids, and sphingolipids exhibited diurnal oscillations, suggesting a widespread circadian impact on muscle lipid metabolism. Notably, peak levels of lipid accumulation were in phase coherence with core clock gene expression in vivo and in vitro. The percentage of oscillating lipid metabolites was comparable between muscle tissue and cultured myotubes, and temporal lipid profiles correlated with transcript profiles of genes implicated in their biosynthesis. Lipids enriched in the outer leaflet of the plasma membrane oscillated in a highly coordinated manner in vivo and in vitro. Lipid metabolite oscillations were strongly attenuated upon siRNA-mediated clock disruption in human primary myotubes. Taken together, our data suggest an essential role for endogenous cell-autonomous human skeletal muscle oscillators in regulating lipid metabolism independent of external synchronizers, such as physical activity or food intake.
Identifiers
PMID: 28973848
Full text
Article (Published version) (2.6 MB) - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://www.pnas.org/content/114/41/E8565
Structures
Research group Endocrinologie et horloge circadienne (948)
Project FNS: CRSII3_154405
Citation
(ISO format)
LOIZIDES-MANGOLD, Ursula et al. Lipidomics reveals diurnal lipid oscillations in human skeletal muscle persisting in cellular myotubes cultured in vitro. In: Proceedings of the National Academy of Sciences, 2017, vol. 114, n° 41, p. E8565-E8574. https://archive-ouverte.unige.ch/unige:98893

10 hits

0 download

Update

Deposited on : 2017-11-13

Export document
Format :
Citation style :