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Scientific article
Open access
English

The genomic silencing of position-effect variegation in Drosophila melanogaster: interaction between the heterochromatin-associated proteins Su(var)3-7 and HP1

Published inJournal of cell science, vol. 113, p. 4253-4261
Publication date2000
Abstract

Position-effect variegation results from mosaic silencing by chromosomal rearrangements juxtaposing euchromatin genes next to pericentric heterochromatin. An increase in the amounts of the heterochromatin-associated Su(var)3-7 and HP1 proteins augments silencing. Using the yeast two-hybrid protein interaction trap system, we have isolated HP1 using Su(var)3-7 as a bait. We have then delimited three binding sites on Su(var)3-7 for HP1. On HP1, the C-terminal moiety, including the chromo shadow domain, is required for interaction. In vivo, both proteins co-localise not only in heterochromatin, but also in a limited set of sites in euchromatin and at telomeres. When delocalised to the sites bound by the protein Polycomb in euchromatin, HP1 recruits Su(var)3-7. Finally, and in contrast with euchromatin genes, a decrease in the amounts of both proteins enhances variegation of the light gene, one of the few genetic loci mapped within pericentric heterochromatin. This body of data supports a direct link between Su(var)3-7 and HP1 in the genomic silencing of position-effect variegation.

Keywords
  • Alleles
  • Animals
  • Chromosomal Proteins, Non-Histone/genetics/metabolism
  • DNA, Satellite/genetics
  • Drosophila Proteins
  • Drosophila melanogaster/genetics
  • Euchromatin/genetics/metabolism
  • Gene Expression/genetics
  • Gene Rearrangement/physiology
  • Gene Silencing
  • Heterochromatin/genetics/metabolism
  • Mutagenesis, Insertional/physiology
  • Repressor Proteins/genetics/metabolism
  • Telomere/genetics/metabolism
  • Two-Hybrid System Techniques
Citation (ISO format)
DELATTRE, Marion et al. The genomic silencing of position-effect variegation in Drosophila melanogaster: interaction between the heterochromatin-associated proteins Su(var)3-7 and HP1. In: Journal of cell science, 2000, vol. 113, p. 4253–4261.
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Article (Published version)
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Identifiers
ISSN of the journal0021-9533
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