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Title

The genomic silencing of position-effect variegation in Drosophila melanogaster: interaction between the heterochromatin-associated proteins Su(var)3-7 and HP1

Authors
Tonka, Chia-Hwa
Published in Journal of Cell Science. 2000, vol. 113, p. 4253-61
Abstract Position-effect variegation results from mosaic silencing by chromosomal rearrangements juxtaposing euchromatin genes next to pericentric heterochromatin. An increase in the amounts of the heterochromatin-associated Su(var)3-7 and HP1 proteins augments silencing. Using the yeast two-hybrid protein interaction trap system, we have isolated HP1 using Su(var)3-7 as a bait. We have then delimited three binding sites on Su(var)3-7 for HP1. On HP1, the C-terminal moiety, including the chromo shadow domain, is required for interaction. In vivo, both proteins co-localise not only in heterochromatin, but also in a limited set of sites in euchromatin and at telomeres. When delocalised to the sites bound by the protein Polycomb in euchromatin, HP1 recruits Su(var)3-7. Finally, and in contrast with euchromatin genes, a decrease in the amounts of both proteins enhances variegation of the light gene, one of the few genetic loci mapped within pericentric heterochromatin. This body of data supports a direct link between Su(var)3-7 and HP1 in the genomic silencing of position-effect variegation.
Keywords AllelesAnimalsChromosomal Proteins, Non-Histone/genetics/metabolismDNA, Satellite/geneticsDrosophila ProteinsDrosophila melanogaster/geneticsEuchromatin/genetics/metabolismGene Expression/geneticsGene Rearrangement/physiologyGene SilencingHeterochromatin/genetics/metabolismMutagenesis, Insertional/physiologyRepressor Proteins/genetics/metabolismTelomere/genetics/metabolismTwo-Hybrid System Techniques
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PMID: 11069770
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DELATTRE, Marion et al. The genomic silencing of position-effect variegation in Drosophila melanogaster: interaction between the heterochromatin-associated proteins Su(var)3-7 and HP1. In: Journal of Cell Science, 2000, vol. 113, p. 4253-61. https://archive-ouverte.unige.ch/unige:9877

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Deposited on : 2010-08-06

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