UNIGE document Scientific Article
previous document  unige:9871  next document
add to browser collection
Title

Position-effect variegation in Drosophila depends on dose of the gene encoding the E2F transcriptional activator and cell cycle regulator

Authors
Spierer, Anne
Szidonya, Janos
Reuter, Gunter
Published in Development. 1996, vol. 122, no. 6, p. 1949-56
Abstract A dominant mutation due to the insertion of a P-element at 93E on the third chromosome of Drosophila melanogaster enhances position-effect variegation. The corresponding gene was cloned by transposon tagging and the sequence of the transcript revealed that it corresponds to the gene encoding the transcriptional activator and cell cycle regulator dE2F. The transposon-tagged allele is homozygous viable, and the insertion of the transposon in an intron correlates with a strong reduction in the amount of transcript. A homozygous lethal null allele was found to behave as a strong enhancer when heterozygous. Overexpression of the gene in transgenic flies has the opposite effect of suppressing variegation. A link is established here, and discussed, between the dose of a transcriptional activator, which controls the cell cycle, and epigenetic silencing of chromosomal domains in Drosophila.
Keywords Amino Acid SequenceAnimalsBase SequenceCarrier ProteinsCell Cycle Proteins/genetics/metabolismCloning, MolecularDNADNA-Binding ProteinsDrosophila ProteinsDrosophila melanogaster/embryology/geneticsE2F Transcription FactorsEnhancer Elements, GeneticEye Color/geneticsFemaleGene DosageGene Expression RegulationInsect HormonesMaleMolecular Sequence DataMutationRNA, Messenger/metabolismRetinoblastoma-Binding Protein 1Trans-Activators/genetics/metabolismTranscription Factors/genetics/metabolism
Identifiers
PMID: 8674433
Full text
Article (Published version) (281 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Citation
(ISO format)
SEUM, Carole et al. Position-effect variegation in Drosophila depends on dose of the gene encoding the E2F transcriptional activator and cell cycle regulator. In: Development, 1996, vol. 122, n° 6, p. 1949-56. https://archive-ouverte.unige.ch/unige:9871

182 hits

0 download

Update

Deposited on : 2010-08-06

Export document
Format :
Citation style :