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Loss of Sendai virus C protein leads to accumulation of RIG-I immunostimulatory defective interfering RNA.

Sánchez-Aparicio, Maria Teresa
Rice, Charles M
García-Sastre, Adolfo
Baum, Alina
Published in Journal of General Virology. 2017, vol. 98, no. 6, p. 1282-1293
Abstract Retinoic acid inducible gene (RIG-I)-mediated innate immunity plays a pivotal role in defence against virus infections. Previously we have shown that Sendai virus (SeV) defective interfering (DI) RNA functions as an exclusive and potent RIG-I ligand in DI-RNA-rich SeV-Cantell infected cells. To further understand how RIG-I is activated during SeV infection, we used a different interferon (IFN)-inducing SeV strain, recombinant SeVΔC, which, in contrast to SeV-Cantell is believed to stimulate IFN production due to the lack of the SeV IFN antagonist protein C. Surprisingly, we found that in SevΔC-infected cells, DI RNAs also functioned as an exclusive RIG-I ligand. Infections with wild-type SeV failed to generate any RIG-I-associated immunostimulatory RNA and this correlated with the lack of DI genomes in infected cells, as well as with the absence of cellular innate immune responses. Supplementation of the C protein in the context of SeVΔC infection led to a reduction in the number of DI RNAs, further supporting the potential role of the C protein as a negative regulator of DI generation and/or accumulation. Our findings indicate that limiting DI genome production is an important function of viral IFN antagonist proteins.
Keywords Adjuvants, Immunologic/metabolismDEAD Box Protein 58/metabolismGene DeletionGene Expression Regulation, ViralGenetic Complementation TestHeLa CellsHumansRNA, Small Interfering/metabolismRNA, Viral/metabolismSendai virus/genetics/immunologyViral Proteins/genetics
PMID: 28631605
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Article (Published version) (3.5 MB) - public document Free access
Research group Virologie moléculaire (275)
Project FNS: 31003A_163129
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SÁNCHEZ-APARICIO, Maria Teresa et al. Loss of Sendai virus C protein leads to accumulation of RIG-I immunostimulatory defective interfering RNA. In: Journal of General Virology, 2017, vol. 98, n° 6, p. 1282-1293. https://archive-ouverte.unige.ch/unige:98539

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Deposited on : 2017-11-03

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