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RNA-protein interactions in the ribosome *1, *2VIII. Co-operative interactions in the 50 S subunit of Escherichia coli

Authors
Zimmermann, Robert A.
Published in Journal of Molecular Biology. 1976, vol. 103, no. 3, p. 647 - 653
Abstract Six 50 S ribosomal subunit proteins, each unable to interact independently with the 23 S RNA, were shown to associate specifically with ribonucleoprotein complexes consisting of intact 23 S RNA, or fragments derived from it, and one or more RNA-binding proteins. In particular, L21 and L22 depend for attachment upon L20 and L24, respectively; L5, L10 and L11 interact individually with complexes containing L2 and L16; and one or both proteins of the L17/L27 mixture are stimulated to bind in the presence of L1, L3, L6, L13 and L23. Moreover, L14 alone was found to interact with a fragment from the 3' end of the 23 S RNA, even though it cannot bind to 23 S RNA. By correlating the data reported here with the findings of others, it has been possible to formulate a partial in vitro assembly map of the Escherichia coli 50 S subunit encompassing both the 5 S and 23 S RNAs as well as 21 of the 34 subunit proteins.
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SPIERER, Pierre, ZIMMERMANN, Robert A. RNA-protein interactions in the ribosome *1, *2VIII. Co-operative interactions in the 50 S subunit of Escherichia coli. In: Journal of Molecular Biology, 1976, vol. 103, n° 3, p. 647 - 653. https://archive-ouverte.unige.ch/unige:9845

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Deposited on : 2010-08-05

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