The goal of this study was to examine the importance of humor as character strength in individuals with Asperger's syndrome/High Functioning Autism (AS/HFA) and how it relates to life satisfaction and orientation to happiness. Thirty-three individuals with AS/HFA and 33 gender-, age- and education-matched typically developing (TD) participants filled out scales assessing character strengths (VIA-IS), life satisfaction (SWLS) and orientation to happiness (OTH). Profile analyses of the character strengths and character strengths factors revealed significant differences between the two groups. Humor was found to be the 8th highest out of 24 character strengths in TD, but was only at the 16th position in individuals with AS/HFA when the strengths are rank-ordered. In TD participants, humor is related to life of pleasure, life of engagement, life of meaning and life satisfaction. In individuals with AS/HFA, humor is only related to life of pleasure. This shows that 1) individuals with AS/HFA seem not to consider humor as one of their important strengths, which is in line with humor difficulties reported earlier and 2) humor does not seem to contribute to life satisfaction to the same degree as in TD controls.