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Title

Methods to Monitor and Quantify Autophagy in the Social Amoeba Dictyostelium discoideum

Authors
Domínguez-Martín, Eunice
King, Jason S
Coria, Roberto
Escalante, Ricardo
Published in Cells. 2017, vol. 6, no. 3
Abstract Autophagy is a eukaryotic catabolic pathway that degrades and recycles cellular components to maintain homeostasis. It can target protein aggregates, superfluous biomolecular complexes, dysfunctional and damaged organelles, as well as pathogenic intracellular microbes. Autophagy is a dynamic process in which the different stages from initiation to final degradation of cargo are finely regulated. Therefore, the study of this process requires the use of a palette of techniques, which are continuously evolving and whose interpretation is not trivial. Here, we present the social amoeba Dictyostelium discoideum as a relevant model to study autophagy. Several methods have been developed based on the tracking and observation of autophagosomes by microscopy, analysis of changes in expression of autophagy genes and proteins, and examination of the autophagic flux with various techniques. In this review, we discuss the pros and cons of the currently available techniques to assess autophagy in this organism.
Identifiers
PMID: 28671610
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Article (Published version) (3.1 MB) - public document Free access
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Research group Groupe Soldati
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DOMÍNGUEZ-MARTÍN, Eunice et al. Methods to Monitor and Quantify Autophagy in the Social Amoeba Dictyostelium discoideum. In: Cells, 2017, vol. 6, n° 3. https://archive-ouverte.unige.ch/unige:96992

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Deposited on : 2017-09-25

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