Scientific article
Open access

Kinase-templated abiotic reaction

Published inChemical science, vol. 8, no. 7, p. 5119-5125
Publication date2017

Protein kinases are quintessential regulators of cellular function. Numerous pathologies are intimately linked to the dysregulated activity of a particular protein kinase. Herein we report a technology based on a proximity-induced chemical transformation that enables the detection and imaging of specific kinases. Using two probes that target the nucleotide-binding site and substrate binding site of a target kinase respectively, the reagents appended on the probes are brought within reactive distance thereby enabling the chemical transformation. The reaction used for sensing is a ruthenium-photocatalyzed reduction of a pyridinium immolative linker, which uncages a fluorophore (rhodamine). We demonstrate that this technology can be used to discriminate between closely related kinases with a high signal to noise ratio. We further demonstrate that the technology operates within the complexity of a cellular context with a good correlation between the level of kinase activity and fluorescence output.

Research group
Citation (ISO format)
SAARBACH, Jacques et al. Kinase-templated abiotic reaction. In: Chemical science, 2017, vol. 8, n° 7, p. 5119–5125. doi: 10.1039/C7SC01416C
Main files (1)
Article (Published version)
ISSN of the journal2041-6520

Technical informations

Creation06/30/2017 6:52:00 PM
First validation06/30/2017 6:52:00 PM
Update time03/15/2023 1:49:10 AM
Status update03/15/2023 1:49:10 AM
Last indexation01/17/2024 12:15:15 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack