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Scientific article
Open access
English

The macro domain as fusion tag for carrier-driven crystallization

Published inProtein science, vol. 26, no. 2, p. 365-374
Publication date2017
Abstract

Obtaining well-ordered crystals remains a significant challenge in protein X-ray crystallography. Carrier-driven crystallization can facilitate crystal formation and structure solution of difficult target proteins. We obtained crystals of the small and highly flexible SPX domain from the yeast vacuolar transporter chaperone 4 (Vtc4) when fused to a C-terminal, non-cleavable macro tag derived from human histone macroH2A1.1. Initial crystals diffracted to 3.3 Å resolution. Reductive protein methylation of the fusion protein yielded a new crystal form diffracting to 2.1 Å. The structures were solved by molecular replacement, using isolated macro domain structures as search models. Our findings suggest that macro domain tags can be employed in recombinant protein expression in E. coli, and in carrier-driven crystallization.

Research group
Funding
  • European Commission - Identity and functions of polyphosphate polymerases in eukaryotes [310856]
Citation (ISO format)
WILD, Rebekka, HOTHORN, Michael. The macro domain as fusion tag for carrier-driven crystallization. In: Protein science, 2017, vol. 26, n° 2, p. 365–374. doi: 10.1002/pro.3073
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Article (Published version)
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ISSN of the journal0961-8368
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Creation06/15/2017 11:28:00 AM
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