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Scientific article
English

Subunit III of bovine procarboxypeptidase A: its relationship to human pancreatic elastase 1

Published inBiological chemistry Hoppe-Seyler, vol. 368, no. 12, p. 1613-1618
Publication date1987
Abstract

This paper is a continuation of our study of various animal pancreatic enzymes which are related to human pancreatic elastase 1 (Sziegoleit, A. & Linder, D. (1986) Biol. Chem. Hoppe-Seyler, 367, 527-531). The isolation and immunological analysis of the related protein from bovine pancreas disclosed that the third subunit of the procarboxypeptidase A complex is the antibody-binding component. The similarity of this subunit to elastase 1 is affirmed by comparison of their primary structures. While the complete amino-acid sequence of bovine subunit III recently has been published (Venot, N., Sciaky, M., Puigserver, A., Desnuelle, P. & Laurent, G. (1986) Eur. J. Biochem. 157, 91-99), we here present the amino-acid sequence of the carboxy-terminal tryptic peptide of human pancreatic elastase 1 showing a high degree of homology.

Keywords
  • Animals
  • Carboxypeptidases/analysis
  • Carboxypeptidases A
  • Cattle
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme Precursors/analysis
  • Humans
  • Immunoassay
  • Isoelectric Focusing
  • Pancreas/enzymology
  • Pancreatic Elastase/analysis
  • Rosaniline Dyes
  • Sodium Dodecyl Sulfate
Affiliation Not a UNIGE publication
Citation (ISO format)
SZIEGOLEIT, A et al. Subunit III of bovine procarboxypeptidase A: its relationship to human pancreatic elastase 1. In: Biological chemistry Hoppe-Seyler, 1987, vol. 368, n° 12, p. 1613–1618.
Identifiers
ISSN of the journal0177-3593
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