Transcriptomic and anatomic parcellation of 5-HT3AR expressing cortical interneuron subtypes revealed by single-cell RNA sequencing

Frazer, Sarah Louise; Prados, Julien; Niquille, Mathieu; Cadilhac, Christelle; Markopoulos, Foivos; Gomez Teijeiro, Lucia; Tomasello, Ugo; Telley, Ludovic; Holtmaat, Anthony; Jabaudon, Denis; Dayer, Alexandre

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Title		Transcriptomic and anatomic parcellation of 5-HT3AR expressing cortical interneuron subtypes revealed by single-cell RNA sequencing
Authors		Frazer, Sarah Louise Prados, Julien Niquille, Mathieu Cadilhac, Christelle Markopoulos, Foivos Gomez Teijeiro, Lucia Tomasello, Ugo Telley, Ludovic Holtmaat, Anthony Jabaudon, Denis Dayer, Alexandre show all authors [1 - 11]
Published in		Nature Communications. 2017, vol. 8, p. 14219
Abstract		Cortical GABAergic interneurons constitute a highly diverse population of inhibitory neurons that are key regulators of cortical microcircuit function. An important and heterogeneous group of cortical interneurons specifically expresses the serotonin receptor 3A (5-HT3AR) but how this diversity emerges during development is poorly understood. Here we use single-cell transcriptomics to identify gene expression patterns operating in Htr3a-GFP+ interneurons during early steps of cortical circuit assembly. We identify three main molecular types of Htr3a-GFP+ interneurons, each displaying distinct developmental dynamics of gene expression. The transcription factor Meis2 is specifically enriched in a type of Htr3a-GFP+ interneurons largely confined to the cortical white matter. These MEIS2-expressing interneurons appear to originate from a restricted region located at the embryonic pallial-subpallial boundary. Overall, this study identifies MEIS2 as a subclass-specific marker for 5-HT3AR-containing interstitial interneurons and demonstrates that the transcriptional and anatomical parcellation of cortical interneurons is developmentally coupled.
Identifiers		DOI: 10.1038/ncomms14219 PMID: 28134272
Full text		Article (Published version) (5 MB) - Free access
Structures		Faculté de médecine / Section de médecine clinique / Département de psychiatrie Faculté de médecine / Section de médecine fondamentale / Département de neurosciences fondamentales
Research group		Groupe Dayer Alexandre (Formation du circuit cortical) (875)
Citation (ISO format)		FRAZER, Sarah Louise et al. Transcriptomic and anatomic parcellation of 5-HT3AR expressing cortical interneuron subtypes revealed by single-cell RNA sequencing. In: Nature Communications, 2017, vol. 8, p. 14219. https://archive-ouverte.unige.ch/unige:94163