T follicular helper expansion and humoral-mediated rejection are independent of the HVEM/BTLA pathway

Rodriguez-Barbosa, Jose-Ignacio; Fernandez-Renedo, Carlos; Moral, Ana María Bravo; Buehler, Leo Hans; Del Rio, Maria-Luisa

doi:10.1038/cmi.2015.101

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Title		T follicular helper expansion and humoral-mediated rejection are independent of the HVEM/BTLA pathway
Authors		Rodriguez-Barbosa, Jose-Ignacio Fernandez-Renedo, Carlos Moral, Ana María Bravo Buehler, Leo Hans Del Rio, Maria-Luisa
Published in		Cellular & Molecular Immunology. 2017, vol. 14, no. 6, p. 497-510
Abstract		The molecular pathways contributing to humoral-mediated allograft rejection are poorly defined. In this study, we assessed the role of the herpesvirus entry mediator/B- and T-lymphocyte attenuator (HVEM/BTLA) signalling pathway in the context of antibody-mediated allograft rejection. An experimental setting was designed to elucidate whether the blockade of HVEM/BTLA interactions could modulate de novo induction of host antidonor-specific antibodies during the course of graft rejection. To test this hypothesis, fully allogeneic major histocompatibility complex-mismatched skin grafts were transplanted onto the right flank of recipient mice that were treated with isotype control, anti-CD40L or modulatory antibodies of the HVEM/BTLA signalling pathway. The frequencies of CD4 T follicular helper (Tfh) cells (B220-, CD4+ CXCR5+ PD-1high), extrafollicular helper cells (B220-, CD4+ CXCR5- PD-1+ and PD-1-) and germinal centre (GC) B cells (B220+Fas+ GL7+) were analysed by flow cytometry in draining and non-draining lymph nodes at day 10 post transplantation during the acute phase of graft rejection. The host antidonor isotype-specific humoral immune response was also assessed. Whereas blockade of the CD40/CD40L pathway was highly effective in preventing the allogeneic humoral immune response, antibody-mediated blockade of the HVEM/BTLA-interacting pathway affected neither the expansion of Tfh cells nor the expansion of GC B cells. Consequently, the course of the host antidonor antibody-mediated response proceeded normally, without detectable evidence of impaired development. In summary, these data indicate that HVEM/BTLA interactions are dispensable for the formation of de novo host antidonor isotype-specific antibodies in transplantation.Cellular & Molecular Immunology advance online publication, 29 February 2016; doi:10.1038/cmi2015.101.
Identifiers		DOI: 10.1038/cmi.2015.101 PMID: 26924526
Full text		Article (Published version) (13 MB) - Limited access to UNIGE Erratum (260 Kb) - Free access
Structures		Faculté de médecine / Section de médecine clinique / Département de chirurgie
Research groups		Chirurgie viscérale (104) Chirurgie viscérale (HUG)
Citation (ISO format)		RODRIGUEZ-BARBOSA, Jose-Ignacio et al. T follicular helper expansion and humoral-mediated rejection are independent of the HVEM/BTLA pathway. In: Cellular & Molecular Immunology, 2017, vol. 14, n° 6, p. 497-510. doi: 10.1038/cmi.2015.101 https://archive-ouverte.unige.ch/unige:93720