Dataset of differential lipid raft and global proteomes of SILAC-labeled cystic fibrosis cells upon TNF -α stimulation

Chhuon, C; Pranke, I; Borot, F; Tondelier, D; Lipecka, J; Fritsch, J; Chanson, Marc; Edelman, A; Ollero, M; Guerrera, I C

doi:10.1016/j.dib.2016.08.012

Scientific article

English

Dataset of differential lipid raft and global proteomes of SILAC-labeled cystic fibrosis cells upon TNF -α stimulation

Contributors

Chhuon, C; Pranke, I; Borot, F; Tondelier, D; Lipecka, J; Fritsch, J; Chanson, Marc

; Edelman, A; Ollero, M; Guerrera, I C

Published in

Data in brief, vol. 9, p. 51-56

Publication date

2016

Abstract

Cystic fibrosis (CF) is a genetic disease due to mutations in the cystic fibrosis transmembrane regulator (CFTR), F508del-CFTR being the most frequent. Lipid raft-like microdomains (LRM) are regions of the plasma membrane that present a high cholesterol content and are insoluble to non-ionic detergents. LRM are essential functional and structural platforms that play an important role in the inflammatory response. CFTR is a known modulator of inflammation in LRM. Here we provide mass spectrometry data on the global impact of CFTR mutation and TNF-a stimulation on the LRM proteome. We used the Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) approach to quantify and identify 332 proteins in LRM upon TNF-a stimulation in CF cells and 1381 for the global proteome. We report two detailed tables containing lists of proteins obtained by mass spectrometry and the immunofluorescence validation results for one of these proteins, the G-protein coupled receptor 5A. These results are associated with the article "Changes in lipid raft proteome upon TNF-α stimulation of cystic fibrosis cells" (Chhuon et al., in press [1]).

UNIGE affiliation entities

Faculté de médecine / Section de médecine clinique / Département de pédiatrie, gynécologie et obstétrique

UNIGE research groups

Mucoviscidose et jonctions GAP (229)

Citation (ISO format)

CHHUON, C et al. Dataset of differential lipid raft and global proteomes of SILAC-labeled cystic fibrosis cells upon TNF -α stimulation. In: Data in brief, 2016, vol. 9, p. 51–56. doi: 10.1016/j.dib.2016.08.012

Article (Published version)

CC BY

Identifiers

PID : unige:93281
DOI : 10.1016/j.dib.2016.08.012
PMID : 27626054

Journal ISSN

2352-3409

471views

192downloads

Creation

20/03/2017 09:10:00

First validation

20/03/2017 09:10:00

Update

15/03/2023 01:33:34

Status update

15/03/2023 01:33:33

Last indexation

31/10/2024 06:40:57

Dataset of differential lipid raft and global proteomes of SILAC-labeled cystic fibrosis cells upon TNF -α stimulation

Contributors

Published in

Publication date

Abstract

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

Main files (1)

Identifiers

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation