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Scientific article
English

Calcium ions are required for the intracellular routing of insulin and its receptor

Published inExperimental cell research, vol. 198, no. 1, p. 144-149
Publication date1992
Abstract

We have studied the role of the cytosolic-free calcium concentration ([Ca2+]i) on the early and later internalization steps of insulin and its receptor. As before, we find that the rate of 125I-insulin internalization in HL60 cells remains normal when [Ca2+]i is lowered 10 times below normal resting level by the use of an intracellular Ca2+ chelator. By contrast, the subsequent intracellular steps, i.e. insulin receptor recycling and insulin degradation, are inhibited in calcium-depleted cells. Under low [Ca2+]i conditions, the association of 125I-insulin with late endosomes and lysosomes is also reduced. This suggests that calcium ions are required for fusion processes occurring at the endosomal or postendosomal stage of internalization. Thus, by regulating insulin receptor recycling and by controlling insulin degradation, Ca2+ ions play a key role in the regulation of insulin action.

Keywords
  • Ammonium Chloride/pharmacology
  • Autoradiography
  • Calcium/ physiology
  • Cell Line
  • Down-Regulation/physiology
  • Endocytosis/drug effects/ physiology
  • Humans
  • Insulin/ metabolism
  • Kinetics
  • Lysosomes/metabolism
  • Microscopy, Electron
  • Radioligand Assay
  • Receptor, Insulin/ metabolism
Citation (ISO format)
CARPENTIER, Jean-Louis, GORDEN, P., LEW, Daniel Pablo. Calcium ions are required for the intracellular routing of insulin and its receptor. In: Experimental cell research, 1992, vol. 198, n° 1, p. 144–149. doi: 10.1016/0014-4827(92)90160-a
Identifiers
ISSN of the journal0014-4827
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