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Scientific article
English

Calcitonin and calcitonin gene-related peptide interact with the same receptor in cultured LLC-PK1 kidney cells

Published inBiochemical and biophysical research communications, vol. 131, no. 2, p. 537-542
Publication date1985
Abstract

Calcitonin and calcitonin gene-related peptide stimulate adenylate cyclase activity and plasminogen activator production in cultured renal tubular LLC-PK1 cells. Salmon [125I]calcitonin and human [125I]calcitonin gene-related peptide bound specifically to the cells. Salmon [125I]calcitonin binding was reduced at lower concentrations of non-radioactive salmon calcitonin than of human calcitonin gene-related peptide. For the stimulation of adenylate cyclase activity and plasminogen activator production, the potency of salmon calcitonin was higher than that of human calcitonin and calcitonin gene-related peptide. In a subclone of LLC-PK cells lacking salmon calcitonin binding sites, no specific binding of [125I]CGRP occurred, and adenylate cyclase activity and plasminogen activator production was not increased by the peptides. Thus, in LLC-PK1 cells the stimulation of adenylate cyclase activity and plasminogen activator production by calcitonin gene-related peptide is probably mediated by the calcitonin receptor.

Keywords
  • Adenylate Cyclase/metabolism
  • Animals
  • Calcitonin/ metabolism/pharmacology
  • Calcitonin Gene-Related Peptide
  • Cell Line
  • Humans
  • Kidney Tubules/drug effects/ metabolism
  • Nerve Tissue Proteins/ metabolism/pharmacology
  • Plasminogen Activators/biosynthesis
  • Receptors, Calcitonin
  • Receptors, Cell Surface/drug effects/ physiology
  • Swine
  • Vasopressins/pharmacology
Citation (ISO format)
WOHLWEND, Annelise Isabelle et al. Calcitonin and calcitonin gene-related peptide interact with the same receptor in cultured LLC-PK1 kidney cells. In: Biochemical and biophysical research communications, 1985, vol. 131, n° 2, p. 537–542.
Identifiers
ISSN of the journal0006-291X
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