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Title

Calcitonin and calcitonin gene-related peptide interact with the same receptor in cultured LLC-PK1 kidney cells

Authors
Malmstrom, K.
Henke, H.
Murer, H.
Fischer, J. A.
Published in Biochemical and Biophysical Research Communications. 1985, vol. 131, no. 2, p. 537-542
Abstract Calcitonin and calcitonin gene-related peptide stimulate adenylate cyclase activity and plasminogen activator production in cultured renal tubular LLC-PK1 cells. Salmon [125I]calcitonin and human [125I]calcitonin gene-related peptide bound specifically to the cells. Salmon [125I]calcitonin binding was reduced at lower concentrations of non-radioactive salmon calcitonin than of human calcitonin gene-related peptide. For the stimulation of adenylate cyclase activity and plasminogen activator production, the potency of salmon calcitonin was higher than that of human calcitonin and calcitonin gene-related peptide. In a subclone of LLC-PK cells lacking salmon calcitonin binding sites, no specific binding of [125I]CGRP occurred, and adenylate cyclase activity and plasminogen activator production was not increased by the peptides. Thus, in LLC-PK1 cells the stimulation of adenylate cyclase activity and plasminogen activator production by calcitonin gene-related peptide is probably mediated by the calcitonin receptor.
Keywords Adenylate Cyclase/metabolismAnimalsCalcitonin/ metabolism/pharmacologyCalcitonin Gene-Related PeptideCell LineHumansKidney Tubules/drug effects/ metabolismNerve Tissue Proteins/ metabolism/pharmacologyPlasminogen Activators/biosynthesisReceptors, CalcitoninReceptors, Cell Surface/drug effects/ physiologySwineVasopressins/pharmacology
Identifiers
PMID: 2996513
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WOHLWEND, Annelise Isabelle et al. Calcitonin and calcitonin gene-related peptide interact with the same receptor in cultured LLC-PK1 kidney cells. In: Biochemical and Biophysical Research Communications, 1985, vol. 131, n° 2, p. 537-542. https://archive-ouverte.unige.ch/unige:9277

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Deposited on : 2010-07-12

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